摘要
目的:探讨从黄根中提取游离总蒽醌的方法,并对提取方法和工艺条件进行优化。方法:选择提取时间、溶剂配比、料液比等影响因素,在单因素实验基础上,采用3因素、3水平的Box-Benhnken响应面分析法进行工艺优化。结果:优化的提取工艺条件为提取时间3.05 h,溶剂配比0.62∶1.00,料液比1.00∶18.83,提取2次,提取温度60℃时,黄根游离总蒽醌的理论提取率最高,可达0.151%。经验证,此条件下黄根游离总蒽醌提取率为0.152%,与理论值较为接近。结论:通过响应面分析法得到的最优工艺,稳定可行,重复性好。
Objective：To extract the total free anthraquinone from Prismatomeria connata and optimize the extraction process. Meth- ods ： The effects of extraction time, solvent volumn ratio and the ratio of material to liquor on extraction technology were studied. Based on the single-factor experiments, a Box-Behnken design（BBD） of three factors at three levels each was applied to optimize the extraction process. Results ： The optimum conditions were as follows ： extraction time ： 3.05 h ; solvent volumn ratio ：0.62 ： 1 ; the ratio of material to liquor： 1 ： 18.83. The highest theoretic extraction rate of the total free anthraquinone in Prismatomeria connata reaches 0. 151% when extracted twice at 60℃. The verification experiments under these conditions show that the yield of total free anthraquinone was 0. 152%, which was in consistency with the theoretic value. Conclusion：A model equation that can be used to predict the experimental results istablished through the response surface method.
出处
《中医学报》
CAS
2017年第5期805-809,共5页
Acta Chinese Medicine
基金
深圳市生物等产业发展专项资金基础研究计划项目(JC201104210142A)
现代古生物和地层学国家重点实验室开放课题基金项目(143115)
关键词
黄根
游离总蒽醌
响应面法
提取工艺
中药
Prismatomeria connate
total free anthraquinone
response surface method
extraction process
Chinese medicine
