VCAM-1和E选择素在血栓闭塞性脉管炎患者血管壁中的表达及其临床意义

Expression and clinical significance of VCAM-1 and E-selectin in thromboangiitis obliterans

目的探讨血管内皮细胞黏附分子-1(VCAM-1)和E选择素(E-selectin)在血栓闭塞性脉管炎(TAO)患者血管壁中的表达及其临床意义。方法取因血栓闭塞性脉管炎行截肢手术的18例患者和因非血管疾病行截肢手术的16例患者的血管组织,采用免疫组织化学法检测组织中VCAM-1和E选择素的表达并与临床资料进行相关性分析。结果TAO患者血管壁中VCAM-1阳性表达率为77.8%(14/18),E选择素阳性表达率为72.2%(13/18),明显高于对照组的6.3%(1/16)和6.3%(1/16),差异均有统计学意义(P<0.01)。VCAM-1和E选择素的阳性率与患者年龄、吸烟量、吸烟时间、药物使用情况没有相关性(P>0.05)。结论 VCAM-1和E选择素的表达与TAO的发生、发展关系密切,检测VCAM-1和E选择素表达可以作为TAO的诊断指标。

Objective To investigate the expression of VCAM-1 and E-selectin in vessel walls of the patients with thromboangiitis obliterans （TAO）. Methods The vessel tissues were taken from 18 cases of amputation operation due to TAO and 16 cases of amputation operation due to non-vascular diseases. The expression of VCAM-1 and E-selectin were assessed by the immunohisto- chemical method. Results The positive expression rate of VCAM-1 in vessel wall of the patients with TAO was 77. 8 % （14/18）, which of E-selectin was 72. 2%（13/18）; both of them were obviously higher than 6. 3%（1/16） and 6. 3%（1/16） in the control group. The positive rate of VCAM-1 and E-selectin had no correlation with the age, smoking amount, smoking time and drug use （P 〉0. 05）. Conclusion The expression of VCAM-1 and E-selectin have close correlation with the occurrence and development of TAO. Detecting the expression of VCAM-1 and E-selectin can serve as a diagnostic indicator of TAO.