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拟南芥铵转运蛋白AtAMT1.3的电生理功能 被引量:1

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摘要 在生理条件下,植物铵的吸收主要由定位于细胞膜上的铵转运蛋白(ammonium transporter,AMT)介导完成,研究模式植物拟南芥AMT的功能特性及调控机制对于解析植物的铵吸收过程具有重要意义。本研究克隆了拟南芥AtAMT1.3并将其在蛙卵异源系统中表达,电生理结果表明,AtAMT1.3是一个典型的对铵有高度选择性、高亲和的铵吸收系统[Km=(25.5±3.2)μmmol/L]。同时,AtAMT1.3也能介导铵的同系物甲基铵的吸收[Km=(3.3±0.2)mmmol/L],对铵和甲基铵的吸收具有浓度依赖性和电压依赖性。AtAMT1.3的铵转运能力不受p H值调控,它的转运底物是NH_4^+,运输机制是NH4+单向运输。
出处 《江苏农业科学》 北大核心 2017年第8期36-40,共5页 Jiangsu Agricultural Sciences
基金 中国科学院战略性先导科技专项(编号:XDB15030202)
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