摘要
对从云南传统发酵豆豉分离得到的植物乳杆菌YM-2(Lactobacillus plantarum YM-2)菌株胞外多糖(exopolysaccharide,EPS)生物合成条件进行优化。首先对培养基成分中的碳源、氮源种类进行筛选;然后利用单因素试验分析碳源含量、氮源含量、培养时间以及培养温度对EPS产量的影响;最后采用Box-Behnken法进行四因素三水平响应面分析以确定其最优工艺条件。结果表明,植物乳杆菌YM-2菌株生物合成EPS最佳条件为碳源(葡萄糖)含量30 g/L、氮源(酵母粉)含量30 g/L、培养时间30.05 h、培养温度36.36℃,在此工艺条件下,植物乳杆菌YM-2 EPS产量为257.362 mg/L。
The fermentation conditions for the production of exopolysaccharides （EPS） by Lactobacillus plantarum YM-2, isolated from traditional fermented tempeh in Yunnan province, were optimized. Firstly, the carbon and nitrogen sources in the medium were screened. Secondly, the effects of carbon source concentration, nitrogen source concentration, culture time and culture temperature on EPS yield were studied through one-factor-at-a-time analysis. Finally, response surface methodology （RSM） based on a four-variable, three-level Box-Behnken design was used to determine the optimal culture conditions as follows： carbon source concentration, 30 g/L; nitrogen source concentration, 30 g/L; culture time, 30.05 h; and culture temperature, 36.36 ℃ Under these conditions, the yield of EPS was 257.362 mg/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第10期24-30,共7页
Food Science
基金
国家自然科学基金地区科学基金项目(31560447)
关键词
植物乳杆菌
胞外多糖
条件优化
响应面法
Lactobacillus plantarum
exopolysaccharides（EPS）
optimization
response surface methodology （RSM）
