UPLC和Q-Exactive高分辨质谱仪联用鉴定大黄鱼中的甘油三酯分子组成

Identification of Triacylglycerols in the Lipids of Pseudosciaena crocea by UPLC and Q-Exactive High-Resolution Mass Spectrometer

应用超高效液相色谱和电喷雾/四极杆-静电场轨道阱的质谱联用技术,分析大黄鱼脂肪中甘油三酯的组成。采用Hypersil Gold C_(18)柱(150mm×4.6mm,2.1μm),流动相为82%甲醇(含0.1%甲酸)溶液和18%异丙醇溶液,混合后添加0.05%乙酸铵溶液,以等度洗脱方式分离大黄鱼脂肪中的甘油三酯组分,柱后流出液采用电喷雾离子源,以正离子模式采集一级质谱分子离子和二级质谱的碎片离子信息,然后与Lipidblast数据库中甘油三酯信息进行比对。研究结果显示,超高效液相色谱和Q-Exactive高分辨质谱仪联用技术快速、准确鉴定出大黄鱼脂质中含有28种甘油三酯。PoOL、Po SO、PPO、PPoO、PSO、MPO、POL、PoSA8种甘油三酯占总量的87.87%以上,其中PoOL(C_(16:1)、C_(18:1)、C_(18:2))相对含量最丰富,占35.261%。

Abstract： The triglyceride composition of Pseudosciaena crocea lipids was analyzed by ultra performance liquidchromatography-electrospray ionization/quadrupole/electxostatic field orbit trap high resolution mass spectrometry （UPLC-ESI/Orbitrap-MS）. The chromatographic separation was performed on a Hypersil Gold C18 column （150 mm × 4.6 mm,2.1 μm） using a mobile phase consisting of methanol （including 0.1% formic acid）-isopropyl alcohol （82：18, V/V） and 0.05%ammonium acetate. The analytes were detected by ESI/Orbitrap-MS in the positive ion mode to obtain mass and tandemmass spectra together and then the results obtained were compared with those from the Lipidblast database. A total of 28triacylglycerols were identified by UPLC-ESI/Orbitrap-MS）. Among these, PoOL was the most abundant （35.261%）, andPoOL, PoSO, PPO, PPoO, PSO, MPO, POL and PoSA together accounted for 87.87% of the total triacylglycerols.