精子DNA碎片化指数2种方法的相关性研究

Comparison of sperm chromatin dispersion test with sperm chromatin structure assay for DNA fragmentation evaluation in human sperm

目的研究精子DNA完整性不同检测方法的区别,探讨使用精子染色质结构分析法(SCSA)和精子染色质扩散实验法(SCD)分析精子DNA碎片化率(DFI)结果的一致性。方法分别用SCSA法和SCD法检测119例不育症患者精液的DFI值,采用SPSS 22.0软件进行相关性分析和配对t检验。结果 SCD法检测119例精液标本DFI均值为(15.4±8.9)%,SCSA法DFI均值为(14.2±7.3)%SCD法DFI值高于SCSA法,差异有统计学意义(P<0.05)。SCSA法和SCD法相关性分析呈明显的线性正相关(r=0.816,P<0.01)。结论 SCSA法与SCD法比较,其检测精子DFI结果具有一致性;但SCD法检测值普遍比SCSA法高。

Objective To assess the difference of different methods in the detection of sperm DNA integrity, and to discuss the consistence of using sperm ehromatin structure assay （ SCSA ） and sperm chromatin dispersion test （ SCD ） in the detection of DNA fragmentation evaluation. Methods We used SCSA and SCD assays to detect DNA fragmentation in sperm from 119 infertile men. The correlation analysis and t - test were performed with SPSS 22.0 software. Results The mean DFI value was （ 15.4 ± 8.9） % by SCD and （ 14.2 ± 7.3） % by SCSA in 119 sperm samples. The SCSA assay was strongly correlated with the SCD assay for sperm DNA fragmentation（ r = 0. 816, P 〈 0.01 ）. However, the mean DIF values with SCD was higher than that with SCSA, with the difference statistically significant（P 〈 0.05 ）. Conclusion There is a strong positive correlation between SCSA and SCD assays in tile detection of DNA fragmentation. SCD assay showed higher levels of DNA fragmentation than that measured by SCSA.