自身免疫性肝炎患者T淋巴细胞活化指标检测价值探讨

Detection value of T lymphocyte activation index in patients with autoimmune hepatitis

目的探讨自身免疫性肝炎(AIH)患者外周血T淋巴细胞表达活化指标HLA-DR、CD25、CD38在AIH发生发展中的机制及临床检测价值。方法应用流式细胞术检测73例AIH组发病期患者、61例AIH组缓解期患者和35例健康对照组外周血T淋巴细胞免疫活化指标(HLA-DR、CD25、CD38),对AIH组发病期、AIH组缓解期与健康对照组的免疫活化指标表达百分率进行比较。结果 AIH组发病期HLA-DR^+、CD3^+HLA-DR^+、CD8^+HLA-DR^+、CD25^+、CD38^+、CD8^+CD38^+表达百分率均高于健康对照组;各组间百分率均数的比较,差异均有统计学意义(P<0.05)。AIH组缓解期HLA-DR^+、CD3^+HLA-DR^+、CD8^+HLA-DR^+、CD25^+、CD38^+、CD8^+CD38^+表达百分率低于发病期,差异均有统计学意义(P<0.05)。结论 T淋巴细胞活化指标HLA-DR、CD25、CD38在AIH发病期表达增加,缓解期表达降低;CD4^+CD25^+在AIH发病期表达降低,缓解期表达增加。

Objective To investigate the mechanism and clinical value of the expression of HLA - DR, CD25, CD38 in the development and progression of AIH in peripheral blood T lymphocytes in patients with autoimInune hepatitis （AIH）. Methods Flow cytometry （FCM） was used to detect the activation markers （HLA- DR, CD25, CD38）of T lymphocytes in peripheral blood of patients in the group of 73 cases of AIH patients in incidence, 61 cases of AIH patients in remission and 35 healthy controls. The expression of the immune activation index in the AIH group, the remission period of the AIH group and the healthy control group were compared. Results The expression of CD3^＋ HLA - DR^＋ , CD8^＋ HLA - DR^＋ , HLA - DR^＋, CD25^＋ , CD38^＋ , CD8^＋ CD38^＋ is higher in AIH incidence group than those in the healthy control, and the difference was statistically significant （P 〈0.05）. The expression of HLA - DR^＋ , CD3^＋ HLA - DR^＋ , CD8^＋ HLA - DR^＋ , CD25^＋ , CD38^＋ , CD8^＋ CD38^＋ in the AIH remission group is lower than the AIH incidence group and the difference was statistically significant （ P 〈 0.05 ）. Conclusion T lymphocyte activation markers HLA - DR, CD25, CD38 increased the incidence period of AIH, while they reduced in remission period ; CD4^＋CD25^＋ reduced the incidence period of AIH, while they increased in remission period.