支原体肺炎患儿免疫功能检测及临床意义

Detection and its clinical significance of immune function in children with mycoplasma pneumonia

目的检测支原体肺炎患儿免疫功能变化情况及临床意义。方法选取2015年1月-2016年1月本院收治的60例支原体肺炎患儿作为研究组,同期在本院体检的60例健康儿童作为对照组,采用流式细胞术检测2组儿童外周血T淋巴细胞亚群;采用免疫透射比浊法检测2组儿童免疫球蛋白IgG、IgA、IgM;采用ELISA法检测细胞因子γ干扰素(IFN-γ)、白介素4(IL-4)及白介素6(IL-6)表达水平。结果研究组患儿的CD3^+、CD4^+及CD4^+/CD8^+指标值、免疫球蛋白IgA及IgM水平、血清IFN-γ、IL-4及IL-6水平均明显高于对照组,差异有统计学意义(P<0.01),而2组儿童CD8^+指标、IgG水平差异无统计学意义(P>0.05)。结论支原体肺炎患儿存在多种淋巴细胞亚群、免疫球蛋白及细胞因子明显异常,导致自身免疫功能紊乱,检测患儿自身免疫功能状况对支原体肺炎患儿的诊断及治疗具有重要参考价值。

Objective To investigate the changes of immune function in children with mycoplasma pneumonia and its clinical significance. Methods 60 cases with mycoplasma pneumonia during January 2015 - January 2016 in our hospital were selected as the study group, and 60 healthy children were selected as control group at the same time. T lymphocyte subsets of peripheral blood were detected by flow cytometry in the two groups; immune globulin IgG, IgA, IgM were detected by immune turbidimet- ric assay; enzyme- linked immunosorbent assay（ELISA） method was used to detect cytokine interferon gamma, interleukin 4 and interleukin 6 expression. Results The levels of CD3^＋ , CD4^＋ and CD4^＋/CD8^＋, IgA and IgM, IFN - 5＇, IL - 4 and IL - 6 in the study group were significantly higher than those in the control group, with the differences statistically significant （ P 〈 0. 01 ） ; while there was no statistical significance on the difference in CD8^＋ and IgG levels between the two groups（ P 〉 0.05 ）. Conclusion There are many abnormalities in subgroups of lymphocytes, immunoglobulins and cytokines in children with mycoplasma pneumonia, leading to autoimmune disorders. Detection of autoimmune function in children with mycoplasma pneumonia has important reference value for the diagnosis and treatment of myeoplasma pneumonia.