鹿血抗氧化活性肽分子量测定及其氨基酸组成分析

Molecular Weight Determination and Amino Acid Composition Analysis of Antioxidant Peptides in Deer Blood

分离提纯了鹿血酶解产物中的抗氧化活性肽,并用高效液相色谱(HPLC)体积排阻法(SEC)测定分子量,分析其氨基酸组成.经SephadexG-25和DEAE-52柱层析纯化,得到了带有较弱负电荷的抗氧化活性肽.HPLC分析结果表明,该抗氧化活性肽为低分子量肽类物质,且是以相对分子量为876,463,146Da为主的小肽混合物.氨基酸组成分析表明,该肽所含17种氨基酸中以亮氨酸、赖氨酸和丙氨酸的质量分数较高,分别为17.2%、12.3%和12.1%,人体必需氨基酸(缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸、蛋氨酸、苏氨酸、赖氨酸)的含量为52.7%.

The antioxidant peptides from enzymolysis of deer blood are isolated and purified, and the size exclusion method （SEC） of high performance liquid chromatography （HPLC） is used to determine molecular weight and to analyze amino acid composition of the peptides. By the isolation and purification of SephadexG 25 and DEAE 52 column, the antioxidant peptides with weak negative charge are obtained. The HPLC results indicate that the antioxidant peptides are small peptide mixture with low molecular weight, mainly including peptides with relative molecular weights of 876 Da, 463 Da and 146 Da. The amino acid composition analysis indicates that the antioxidant peptides contain 17 kinds of amino acids in which Leu, Lys and Ala contents are higher, i.e., 17.2%, 12.3% and 12.1% respectively, and the content of amino acids （Val, Ile, Leu, Phe, Met, Thr and Lys） essential to human body is up to 52.7%.