146株致泻性大肠埃希菌多重PCR结果分析

Analysis on the multiple PCR results of 146 strains of Diarrheagenic E.coli

目的了解福建省致泻性大肠埃希菌分离株的流行病学特征及毒力基因携带情况,为防控感染性腹泻暴发提供客观的流行病学资料。方法 2010年6月至2016年10月通过致泻性大肠埃希菌多重PCR和单重PCR检测方法确定其致病型别,并分析其毒力基因分布。结果 2 231份粪便标本中共分离到146株致泻性大肠埃希菌,阳性率为6.54%;其中4株EPEC均为escV(+)、bfpB(+)、stx1(-)、stx2(-),65株aEPEC为escV(-)、bfpB(+)、stx1(-)、stx2(-),39株EAEC中,35株aggR(+)、3株pic(+)、1株aggR(+)、pic(+),38株ETEC中19株elt(+)、14株estIa(+)、5株elt(+)、estIa(+);男80例,女66例,<3岁的57例,占39.14%,>45岁的51例,占34.93%。结论福建省存在致泻性大肠埃希菌感染性腹泻病例,以婴幼儿和中老年为主要病例构成;致病型别以aEPEC为主,EAEC、ETEC次之,未检出EIEC、EHEC;相关毒力基因呈多态性携带情况,应加强其他感染性腹泻病例中5种致泻性大肠埃希菌监测。

Objective To understand the epidemiological characteristics and virulence gene of Diarrheagenic E. coli isolates in Fujian province,so as to provide the basis for the prevention and control of infectious diarrhea ountbreak. Methods The multiple PCR and single PCR detection methods were used to determine its pathogenic caused by Diarrheagenic E. coil,during June 2010-October 2016 ,and analyze the distribution of virulence genes. Results In a total of 2 231 stool specimens, 146 strains Diarrheagenic E. coli were isolated,the positive rate was 6.54% ;Four EPEC strains were carrying escV （＋）,bfpB （＋）,stxl （ -）,stx2 （- ）,65 strains aEPEC were carrying escV （-）,bfpB （＋）,stxl （-）,stx2 （ ）,39 strains were carrying EAEC ,35 strains of aggR （＋） ,3 strains of PIC （-） ,1 strain aggR （＋） and PIC （＋） ,38 strains of ETEC 19 strains of elt （ ＋ ）, 14 strains estla （ - ）, 5 strains of elt （ ＋ ）, estIa （ ＋ ） ; （80 male and 66 female, 57 cases under 3 years old （39. 14%）,51 cases above 45 years old, accounting for 34.93 %. Conclusion E. coli infective diarrhea cases exist in Fujian province, mainly among the babies and infants and the elderly;The pathogenic type is mainly aEPEC, followed by EAEC and ETEC ,no EIEC and EHEC were detected;The related virulence genes are in polymorphism carrying state,monitoring should be strengthened on the other 5 kinds of Diarrheagenic E. coli in other infectious diarrhea ca ses.