响应面法优化羌活-独活药对提取工艺

Optimization of Ultrasonic-assisted Extraction of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix Using Response Surface Methodology

目的:采用响应曲面法对羌活-独活药对的超声辅助提取工艺进行优化。方法:在单因素试验基础上,采用HPLC法测定蛇床子素、异欧前胡素含量,并以蛇床子素、异欧前胡素得率的综合评分为指标,通过响应面法考察超声时间、甲醇体积分数、料液比、提取次数对羌活-独活混合提取工艺的影响。结果:响应面法超声提取羌活-独活药对中蛇床子素和异欧前胡素的最佳工艺参数:超声时间40 min,甲醇体积分数95%,料液比1∶15,提取次数为2次。在此条件下,蛇床子素和异欧前胡素的得率分别为(2.113±0.096)mg·g-1、(1.884±0.082)mg·g-1,均与理论预测值吻合。结论:该响应面法优选的提取工艺稳定可行,可用于药对羌活、独活中蛇床子素和异欧前胡素的提取。

Objective：Response surface methodology was used to optimize the extraction conditions of Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix. Methods：Based on one factor test,with composite score of extract amounts of osthole and isoimperatorin as index,response surface methodology was adopted to investigate effects of ultrasonic time,methanol volume fraction,material/liquid ratio and extraction times on extraction process. Results：The optimal extracting parameters of osthole and isoimperatorin from Notopterygii Rhizoma et Radix and Angelicae Pubescentis Radix by response surface methodology were as follows：ultrasonic time 40 min,methanol volume fraction 95%,material/liquid ratio 1∶15 and extraction twice. Under this condition,osthole and isoimperatorin were（2.113±0.096）mg·g^（-1）and（1.884±0.082）mg·g^（-1）,respectively,which are in agreement with the theoretical predictions. Conclusion：This optimized extraction process was stable and feasible,it could be used to extract osthole and isoimperatorin.