摘要
为获得抗大肠杆菌Iss蛋白的抗体,将纯化的GST-Iss蛋白分别免疫雏鸡及小鼠。结果显示,无免疫佐剂、弗氏佐剂免疫雏鸡所产生的Iss抗血清效价仅为1∶100,氢氧化铝佐剂免疫小鼠所产生的Iss抗血清效价为1∶60 000。Iss融合蛋白采用氢氧化铝佐剂免疫小鼠,可获得更强的体液免疫应答,抗体效价更高。
In order to obtain the antibody against the Iss protein of E. coli,the purified GST-Iss protein was immunized to chicken and mouse respectively. The results showed that Iss antiserum titer of the immune adjuvant-free,Freund's adjuvant immu-nized chicken was just 1:100,the aluminum hydroxide adjuvant immunized mice generated Iss titer of antiserum to 1:60000. The Iss fusion protein using aluminum hydroxide adjuvant immunized mice can obtain stronger humoral immune responses,an-tibody titer was higher.
出处
《湖北农业科学》
2017年第7期1307-1309,共3页
Hubei Agricultural Sciences
基金
国家自然科学基金青年项目(31302128)