袋鼠传染性腹泻病的病原分离与鉴定

Isolation and Identification of the Infectious Diarrhea Disease Pathogen in Kangaroo

袋鼠(Macropus)传染性腹泻病在上海野生动物园先后发生过5次,平均发病率为32.7%,致死率为72.9%,造成了巨大损失。通过总结每次发病的流行病学调查结果、临床诊查特征、病理剖检情况、实验室分离鉴定以及动物实验结果,确诊引起本病的病原为大肠埃希氏菌(Escherichia coli),并根据菌体O抗原鉴定为O9菌株和O79菌株。由于此大肠杆菌对许多抗菌药不敏感,或对暂时敏感的抗生素也很快产生耐药性,导致治疗效果极差。因此通过此次病原的分离确诊,得以用制作自家苗的免疫方法,预防了袋鼠传染性腹泻病的再次发生。

There have been five outbreaks of infectious diarrhea among Kangaroo at Shanghai Wild Animal Park and these caused enormous losses. The morbidity and mortality due to diarrhea were 32.7% and 72.9%, respectively. By summarizing the epidemiological survey, clinical symp- toms, pathological alteration, laboratory examination and animal experiment of each outbreak, we confirmed that the diarrhea was caused by pathogenic Escherichia coil that we identified as the 09 strain and 079 strain according to the bacteria O antigen test. The treatment effect was very poor because this E. coli strain is resistant to many antibiotics, or temporarily sensitive to some antibi- otics but soon resistant. We formulated our own vaccine by isolation of the pathogeny to protect kangaroos from infectious diarrhea.