佳木斯地区MTHFR基因多态性与缺血性脑卒中的关系分析

The analysis of polymorphism relationship of MTHFR gene and ischemic stroke in jiamusi

目的:探讨佳木斯地区亚甲基四氢叶酸还原酶基因多态性与缺血性脑卒中的关系。方法:选取缺血性脑卒中患者181例为实验组,健康体检者169例为对照组。通过荧光原位杂交法检测实验组和对照组MTHFR C677T、A1298C位点基因多态性。结果:实验组和对照组MTHFR C677T CC、CT、TT基因型比例分别为17.68%/20.71%、37.02%/47.34%、45.30%/31.95%,两组差异有显著性(P<0.05);MTHFR A1298C AA、AC、CC基因型比例分别为58.56%/58.58%、37.02%/38.46%、4.42%/2.96%,差异没有显著性(P>0.05)。结论:MTHFR C677T基因多态性与缺血性脑卒中具有相关性,T等位基因可能是缺血性脑卒中的易感基因;MTHFR A1298C基因多态性与缺血性脑卒中没有关系。

Objective： To analyze polymorphism relationship of methylenetetrahydrofolate reductase（MTHFR） gene and isehemic stroke patients in Jiamusi. Methods： Total 181 cases of ischemic stroke patients were enrolled as experimental group, and 169 healthy cases were enrolled as control group. MTHFR C677T、A1298C genotypes were detected by the Fluorescence by situ hybridization method. Results： In the experimental group and control group, MTHFR C677T genotype proportions were CC for 17.68%/20.71%, CT for 37.02%/47.34% , TT for 45. 30%/31. 95%. Compared experimental group with control group, there was a significant difference （P 〈 0. 05） ; and MTHFR A1298C genotype proportions were AA for 58.56%/58. 58% ; AC for 37.02%/ 38.46% ; and CC for 4.42%/2.96%. The difference was not statistically significant （P 〉 0. 05）. Conclusion： MTHFR C677T polymorphism and ischemic stroke are relevant. T allele may be a susceptible gene for ischemic stroke. MTHFR A1298C polymorphism is not associated with ischemic stroke.