茉莉酸受体多功能纳米探针的合成与应用

Synthesis and Application of Multifunctional Nanoprobe of Jasmonic Acid Receptor

将巯基丙酸修饰的CdTe量子点和植物激素茉莉酸通过一步法偶联到表面修饰氨基的Fe_3O_4纳米颗粒表面,合成了一种同时具有荧光信号、磁响应性能和特异性标记植物原生质体中茉莉酸受体的多功能磁性荧光纳米探针.荧光光谱测试表明该探针保持了CdTe量子点荧光性能,具有荧光强度高、适用体系的pH范围宽和抗光漂白性能好等优点;磁响应及磁滞回线测试充分说明该探针具有良好的超顺磁性.使用该探针对绿豆原生质体中茉莉酸受体进行成像,结果表明:该探针对茉莉酸受体具有选择性标记的功能,茉莉酸受体主要存在于原生质体膜上.该标记方法简单方便,能为茉莉酸信号转导研究提供可视化的信息.

Mercaptopropionic acid modified CdTe quantum dots and jasmonic acid （JA） were coupled to the sur- face of Fe3 O4 nano particle, and magnetic fluorescent nanoprobe with high fluorescence properties, strong magnetic response and specific labeling of JA receptor was synthesized. The fluorescence spectra proved that the probe main- tained the superior fluorescence properties of CdTe quantum dots such as high fluorescence intensity,wide pH range of application, good anti-photobleaehing properties. The hysteresis loop test shown the probe had good magnetism response performance. The probe was then utilized to image the JA receptor in protoplast of mung bean. The results indicated that this probe had high selectivity to the JA receptor and the JA receptor was mainly detected in the pro- toplast membrane. The labeling approach was convenient and can offer visual information for the signal transduction study of JA.