SEPT11基因在小鼠睾丸精子发生过程中的表达特征

Expression characteristics of the SEPT11 gene in the mouse testis during spermatogenesis

目的初步探讨Septin11(SEPT11)基因在精子发生中的作用。方法通过实时定量PCR、蛋白免疫印迹及免疫荧光等方法检测SEPT11在不同周龄野生型小鼠以及成年睾丸支持细胞激素受体(Ar)特异性敲除小鼠(SCARKO)和Ar全敲除(ARKO)小鼠睾丸中的表达特征。结果 SEPT11基因小鼠出生2周内高表达,随后表达水平降低,出生6周后出现并与未释放的成熟精子共定位且高表达。与野生型小鼠相比,SEPTIN11在SCARKO小鼠和ARKO小鼠睾丸中表达降低(P<0.01),m RNA水平显著升高(P<0.01);SEPTIN11在成熟精子中定位于尾部。结论 SEPT11基因在小鼠出生时表达最高;SEPTIN11在小鼠成熟精子中定位于尾部;Ar的敲减提高了SEPT11的表达。

Objective To investigate the expression characteristic of SEPT11 in mouse testis and its role in spermatogenesis. Methods Real-time PCR, Western blotting and immunofluorescence were applied to study the expression characteristics ofgene SEPT11 in mouse testis at different postnatal week and from Sertoli cell-selected Ar knockout mice （SCARKO） and Ar knockout （ARKO） adult mice. Results The expression of SEPT11 was highest during postnatal 2 weeks, and then fall down. SEPTIN11 colocalized with mature sperm attached with Sertoli cells in the testis from mice at postnatal 6 weeks. The expression of SEPTIN11 in SCARKO and ARKO mice testis were much lower compared with the wild type （P〈0.01）, the mRNA level was significantly higher （P〈0.01）. SEPTIN11 localized to the tail of mature sperm collected from epididymis. Conclusion The new born mice had the highest expression level of SEPT11. SEPTIN11 localized to the tail of mature sperm. Knock-down of Ar increased the expression of SEPT11.