逍生散对干眼杯状细胞影响的实验研究

Effects of Xiaosheng San on goblet cells of dry eyes in mice: a laboratory study

目的通过C57BL/6小鼠干眼模型,探讨中药复方逍生散对干眼杯状细胞的影响及治疗干眼的机制。方法将180只健康C57BL/6小鼠随机分6组:分别为正常组,模型组,生理盐水组,逍生散低剂量、中剂量、高剂量组,每组30只。每天于小鼠后肢根部,皮下注射氢溴酸东莨菪碱造成干眼模型。造模成功后于造模前、造模后、治疗后第4周,测定其泪膜破裂时间、泪液分泌量、角膜染色、结膜杯状细胞数量等情况。结果东莨菪碱皮下肌肉注射造成小鼠干眼模型成功,造模后模型组、生理盐水组、逍生散低剂量组、逍生散中剂量组、逍生散高剂量组在泪液分泌、泪膜破裂时间、杯状细胞数量方面均有明显下降,角膜荧光染色具有明显增加,与造模前有差异(P<0.05),正常组与造模前无明显改变,无统计学意义(P>0.05)。治疗后逍生散中剂量组、逍生散高剂量组在泪液分泌、泪膜破裂时间、角膜荧光染色、杯状细胞数量方面均有明显改善,具有统计学意义(P<0.05)。逍生散低剂量组在泪膜破裂时间有所延长,角膜荧光染色分数有所下降,具有统计学意义(P<0.05)。正常组、模型组、生理盐水组在治疗后有所改变,不具有统计学意义(P>0.05)。结论东莨菪碱皮下肌肉注射可造成小鼠干眼模型。逍生散可增加小鼠泪液分泌量,延长泪膜破裂时间,提高泪膜的稳定性,减少杯状细胞的凋亡,减少眼表损伤等。

Objective To study the effects of Xiaosheng San（ XSS,Xiaosheng Granules） on goblet cells of dry eyes in mice and evaluate its mechanism. Methods 180 male C57BL/6 mice were divided into six groups： normal control（ group A）,model（ group B）,saline（ group C）,XSS low-dose（ group D）,XSS medium-dose（ group E） and high-dose（ group F） groups. Dry eye model was established by injecting scopolamine subcutaneously at the base of rear legs in all groups except normal control group.Schirmer I test（ SIT）,tear break-up time（ BUT）,corneal fluorescent（ FL） staining,and the number of conjunctival goblet cells before and after modeling,and at four weeks after intervention were measured.Results BUT,SIT,and number of goblet cells in group B,C,D,E,and F were significantly decreased after scopolamine treatment（ P〈0. 05）,while no significant difference（ P〉0. 05） was found in group A. FL were significantly increased after model establishment（ P〈0. 05）. Medium-and high-dose XSS significantly improved SIT,BUT and the number of goblet cells in group E and F（ P〉0. 05）. No such changes were observed in group A,B and C（ P〉0. 05）. Conclusion Subcutaneous injecfion of scopolamine could effectively establish dry eye model in mice. Xiaosheng Granules could increase tear secretion,prolong tear break-up time,improve stability,inhibit apoptosis of goblet cells and reduce injury to eye surface.