摘要
目的探讨抑制性细胞因子IL-35、IL-10和TGF-β在女性妊娠期和非妊娠期的表达水平.以及IL-35与复发性自然流产的相关性。方法通过酶联免疫吸附测定法分析120例正常妊娠、40例复发性自然流产史孕妇、40例产后健康妇女和40例非妊娠妇女的IL-35、IL-10、TGF-β的血清水平。相关性分析采用单因素logistic回归分析。结果健康妊娠女性血清IL-35水平显著高于非妊娠女性[333.6(59.32,1391)pg/ml VS 123.9(8.763,471.7)pg/ml;P〈0.001]。与非妊娠女性相比,TGF-β在妊娠早期显著升高[473.4(398.0,580.5)p∥mlvs379.7(311.0,441.3)pg/ml,P〈0.01]。血清IL-10水平在妊娠前后差异无统计学意义[8.602(5.854,12.89)pg/rnlVS9.339(5.691.12.07)pg/ml;P〉O.051。与健康妊娠早期的孕妇相比,复发性自然流产史孕妇的血清IL-35水平显著降低[220.4(4.951,702.0)pg/ml VS 386.5(64.37,1355)pg/ml;P〈O.05]。IL-35与习惯性流产的发生呈负相关(回归系数=-0.003,OR=0.997)。结论IL-35的血清水平在正常妊娠孕妇中增加,在复发性自然流产史孕妇中降低。IL-35是维持母胎免疫耐受的主动参与者,而不是IL-10或TGF-β。
Objective To analyze the levels of IL-35, IL-10 and TGF-β in women with or without pregnancy and to investigate the correlation between |L-35 and recurrent spontaneous abortion. Methods Levels of IL-35, IL-10 and TGF-β in serum were analyzed by enzyme-linked immunosorbent assay (ELISA) in 120 gravidas with normal pregnancy, 40 gravidas with a history of recurrent spontaneous abortion, 40 healthy postpartum women and 40 healthy non-pregnant women of childbearing age. Single factor logistic re- gression analysis was used for correlation analysis. Results The level of serum IL-35 in normal pregnancies was significantly higher than that in non-pregnant women [333.6 (59.32, 1 391 ) pg/ml vs 123.9 (8. 763, 471.7) pg/ml, P〈O. 001 ]. The level of TGF-βincreased significantly in the first trimester as compared with that in non-pregnant women [473.4 (398.0, 580.5) pg/ml vs 379.7 (311.0, 441.3) pg/ml, P〈 O. 013. No significant difference in the level of serum IL-10 was observed between gravidas and non-gravidas [ 8. 602 (5. 854, 12.89) pg/ml vs 9. 339 (5. 691, 12.07) pg/ml, P〉0.05 ]. The level of serum IL-35 in gravidas with recurrent spontaneous abortion was significantly lower than that in healthy gravidas in their first trimester [220.4 (4. 951, 702.0) pg/ml vs 386.5 (64.37, 1 355) pg/ml, P〈O. 05 ]. Serum IL-35 was negatively correlated with the occurrence of recurrent spontaneous abortion ( regression coefficient = -0.003, OR=0.997). Conclusion The level of serum IL-35 increases in healthy gravidas, but decreases in gravi- das with recurrent spontaneous abortion. IL-35, rather than IL-10 or TGF-β, is recognized as an active play- er in maternal-fetal immune tolerance.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2017年第4期245-249,共5页
Chinese Journal of Microbiology and Immunology
基金
上海市卫计委青年项目(20154Y0029)