小黄鱼内脏蛋白的提取及应用研究

Study on Purification of Protease from Pseudosciaena polyactis Offal and Its Application

本文以小黄鱼内脏为研究对象,从中分离提取出活性蛋白。通过对缓冲液p H,提取时间,醇液比3个单因素的考察,确定因素水平,并设计正交试验,对工艺进行优化,得出最佳提取工艺。试验结果表明最佳提取工艺为:提取时间5 h,缓冲液p H9,醇液比0.8∶1(体积比),在此工艺下所提取的蛋白液浓度为3.409 mg/m L。最后,用所提取的活性蛋白进行初步应用性试验,通过美拉德反应,制备食品添加剂,为小黄鱼下脚料的应用提供了理论基础。

The small yellow croaker visceral as the research object,designing the extraction process,separating and extracting the active protein from the small yellow croaker visceral. Determining the factor level through the investigation of three single factor,buffer pH,extraction time and alcohol liquid,to optimize the process and obtain the best extraction process by designing the orthogonal experimental L9（3^3）. The results showed that：5 h of extraction time,9 of buffer pH,0.8 ∶ 1（volume ratio）of alcohol liquid ratio made the best extraction,in this process the extracted an average of 3.409 mg/m L. Finally,with the extraction of active protein applied experiments,through maillard reaction,the preparation of food additives,provides theoretical basis for the application of small yellow croaker scraps.