固相萃取超高效液相色谱法测定食品中非法添加色素红2G

Determination of Red 2G in Food by Solid Phase Extraction-Ultra High Performance Liquid Chromatography

建立快速测定食品中的非法添加色素红2G的固相萃取超高效液相色谱分析方法。选取可能添加红2G的香肠、红酒、红醋、糖果、年糕、牛肉干、海鲜酱、辣椒酱、胡椒粉、辣椒粉、辣椒油、火锅底料作为分析对象,样品用50%(体积分数)甲醇水溶液提取,经固相萃取小柱净化,以含乙酸(0.02 mo L/L)的乙酸铵(0.01 mo L/L)水溶液和甲醇为流动相,经C18色谱柱分离,二极管阵列检测器检测,检测波长508 nm。红2G在0.2 mg/L^100 mg/L浓度范围内呈良好的线性关系,相关系数为1.000 0,方法检出限为0.03 mg/kg,样品平均回收率在80%以上,相对标准偏差小于5%(n=6)。该方法灵敏度高,精密度好,分析时间短,适用于食品中非法添加色素红2G的检测。

A method based on solid phase extraction-ultra high performance liquid chromatography for rapid determination of red 2G in food was established. Taking sausage, red wine, red vinegar, candy, rice cake, beef jerky, hoisin sauce, chilli sauce, pepper, paprika, capsicol and hotpot condiment in which red 2G probably was added as research objects, red 2G in samples was extracted by 50 % （volume fraction）methanol aqueous solution and then purified with a solid phase extraction column. Red 2G was separated by a C18 column （ 100 mm×3.0 mm, 1.7 μm） with methanol and ammonium acetate （0.01 moL/L） containing acetic acid （0.02 moL/L） as mobile phase. The detection wavelength was 508 nm and a good linear relationship was achieved over the range of 0.2 mg/L to 100 mg/L with a correlation coefficient of 1.000 0. The detection limit was 0.03 mg/kg and the average recovery for spiked samples was over 80 % with the relative standard deviation less than 5 % （n=6）. The method possessed high sensitivity, good precision and shorter analysis time, suitable for fast determination of red 2G in food.