中成药六味地黄丸对急性肾损伤保护作用的研究

Study on Protective Effect of Chinese Patent Medicine Liuwei Dihuang Pill on Acute Kidney Injury

利用代谢组学方法研究了六味地黄丸治疗庆大霉素诱导的急性肾损伤的作用机制,并将超高效液相色谱-质谱联用(UPLC-MS)技术与主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)相结合来研究正常组、模型组、给药组间生物标记物的变化情况。在PCA与OPLS-DA模型中,正常组、模型组、给药组均可明显区分。共鉴定出17个正常组与模型组间的生物标记物,包括多种磷脂酰胆碱、鞘氨醇、植物鞘氨醇、鞘磷脂(d18∶0/16∶1(9z))、花生四烯酸。与正常组相比,鞘氨醇、植物鞘氨醇、鞘磷脂(d18∶0/16∶1(9z))、花生四烯酸的含量升高,而给药组中这4种化合物的含量下降,因此六味地黄丸可能通过调节这4种生物标记物的含量从而达到治疗庆大霉素诱导的急性肾损伤的目的。该研究为临床上六味地黄丸治疗肾损伤提供了理论依据。

Metabonomics technology was used to investigate the mechanism of Liuwei Dihuang Pill treating acute kidney injury induced by gentamicin. The ultra performance liquid chromatography - mass spectrometry （UPLC - MS ） coupled with principle component analysis （PCA） and orthogonal partial least squares discriminant analysis （ OPLS - DA） were used to evatuate the changes of biomark- ers of control group, model group and treatment group. Significant differences between the control group, model group and treatment group were observed in clustering analysis, PCA and OPLS -DA model. Seventeen potential biomarkers between control group and model group were identified as mul- tiple phosphatidycholines, sphinganine, phytosphingosine, SM（dl8 ： 0/16 ： 1（9z））, arachidonic acid. The relative contents of sphinganine, phytosphingosine, SM （ d18 ： 0/16 ： 1 （ 9z ） ） and arachidonic acid increased in model group compared with control group, while decreased in treatment group. Therefore, Liuwei Dihuang Pill may achieve the aim to treat acute kidney injury induced by gentamicin through adjusting the content of these biomarkers. This study provides a theoretical basis for Liuwei Dihuang Pill treating kidney injury in clinical practice.