核磁共振氢谱法测定甘露聚糖肽中多糖的含量

Determination of Polysaccharide Content in Mannatide by ~1H-NMR

以甘露聚糖肽为研究对象,首次采用1H-NMR法测定多糖产品中的多糖含量。以基准试剂邻苯二甲酸氢钾为内标,考察了弛豫延迟时间和采样次数对测定结果的影响。选定核磁共振参数弛豫延迟时间1 s,采样次数16次。考察结果表明,该方法具有很好的重复性和精密度。在对甘露聚糖肽定性鉴定的基础上,采用绝对定量方式测定了甘露聚糖肽实际样品的多糖含量,结果与苯酚-硫酸法一致。该方法专属性高,操作方便,结果准确,不仅可用于甘露聚糖肽中多糖含量的测定,还可用于其它结构明确的多糖含量测定。

A proton-nuclear magnetic resonance（1H -NMR） method was established for the determi- nation of polysaccharide content in mannatide using potassium acid phthalate as the internal standard. Effects of some experiment parameters were investigated. The optimal conditions were selected as fol- lows： relaxation delay time（all）： 1 s; transient number（ns） ： 16. The results indicated that, under the optimal conditions, the good precision and repeatability for the method were obtained. The polysaccharide content in mannatide, determined by the 1H -NMR method with inner standard model, was similar to that of the ultraviolet spectrophotometry method. The established method was simple, specific and repeatable. Therefore, it could be used not only for the determination of polysaccharide content in mannatide, but also for detection of other kinds of polysaccharide with definite structures.