阳和汤抑制阳虚证小鼠Lewis肺癌生长的实验研究

Experimental Study of Yanghe Decoction(阳和汤) on Inhibiting Lewis Lung Carcinoma in Mice of Yang Deficiency Syndrome

目的:研究阳和汤对阳虚证Lewis肺癌小鼠抑瘤率、NK细胞活性、Bax、Bcl-2、VEGF水平的影响。方法:将40只小鼠随机分为阳虚Lewis组、阳和汤临床等效剂量组、阳和汤2倍临床等效剂量组、顺铂组,每组10只。采用氢化可的松注射液肌肉注射制作阳虚证小鼠模型,然后将Lewis肺癌细胞株接种于小鼠右侧腋部皮下,造模1周后开始给药。末次给药24 h后处死小鼠,取瘤称重计算抑瘤率,采用乳酸脱氢酶释放实验检测小鼠脾脏NK细胞活性,取瘤组织分别测定Bcl-2、Bax、VEGF的表达。结果:阳和汤对阳虚证Lewis肺癌小鼠肿瘤生长具有抑制作用,能恢复阳虚证Lewis肺癌小鼠脾脏NK细胞活性,有效降低阳虚证Lewis肺癌小鼠Blc-2表达,提高Bax表达,并且能降低VEGF表达。结论:阳和汤对于阳虚证肺癌的生长具有明确抑制作用,其作用强度与剂量有关,其抗肿瘤的可能机制是增加NK杀伤细胞活性、调节Bax、Bcl-2表达水平和抑制肿瘤血管生成。

Objective： To study the influence of Yanghe Decoction on inhibitory rate, NK cell activity, Bax, Bel-2 and VEGF level in Lewis lung cancer mice of Yang deficiency syndrome. Methods： The 40 mice were randomly divided into Lewis group, Yanghe Decoction clinical equivalent dose group, Yanghe Decoction 2 times clinical equivalent dose group, and DDP group, l0 mice in each group. Hydrocortisone Injection intramuscular injection was applied to make Yang deficiency syndrome mice model, then Lewis lung cancer cells were inoeulated into the right axillary subcutaneous of mice. The mice were given medicine after the modeling a week. The mice were sacrificed and the tumor inhibition rate was calculated, 24 h after the last administration. The expression of Bcl-2, Bax and VEGF were determined by using lactate dehydrogenase release assay and NK cell activity in mice spleen. Results： Yanghe Decoction can inhibit the growth of Lewis lung cancer in miee with Yang deficiency syndrome, recover the spleen NK cell activity, effectively reduce Ble-2 expression, increase Bax expression, and decrease the expression of VEGF. Conclusion： Yanghe Decoction can inhibit the growth of Yang deficiency syndrome lung cancer; the therapeutic effect is related to the dosage of the drug; the possible anti-tumor mechanism is to increase the NK killer cell activity, regulating Bax and Bcl-2 expression and inhibition of tumor angiogenesis.