陕产大黄结合型蒽醌转化游离型蒽醌的发酵条件优化

Optimization of Fermentation Conditions for Conversion of Conjagated Anthraquionone into Free Anthraquionone in Dahuang(Radix et Rhizoma Rhei) from Shaanxi

目的:利用正交试验优选大黄结合型蒽醌转化为游离型蒽醌的发酵条件。方法:以大黄中游离型蒽醌的转化率为指标,以发酵时间,发酵温度,摇床转速,装液量为考察因素,正交试验选择较优发酵条件。结果:当发酵温度为35℃,发酵时间为7 d,装液量50 m L,摇床转速为140 r/min时,游离型蒽醌的转化率可达到42.369%。结论:酵母菌发酵能够使大黄中的结合型蒽醌有效地转化为游离型蒽醌,从而降低大黄的峻泻作用。

Objective： Conjagated anthraquionone is the main composition in Dahuang （Radix et Rhizoma Rhei）. To study the Fermentation conditions for conversion of eonjagated anthraquionone into free anthraquionone by orthogonal array design. Methods： Optimization by orthogonal array design based on free anthraquionone conversion rate, and desorption capacity were compared with different types of fermentation temperature, fermentation time, fluid volume, table speed. Results： The optimal conditions of fermentation the was fermentation temperature 35 ℃; fermentation time was 7 d; fluid volume was 50 mL; table speed was 140 rad/min; in these condition, the free anthraquionone conversion rate was 42.369%. Conclusion： Fermentation is an effective way to onversion of eonjagated anthraquionone into free anthraquionone, and the side effeet of severe diarrhea caused by Dahuang （Radix et Rhizoma Rhei） can be alleviated through fermentation processing by yeast.