北京市健康从业人员携带金黄色葡萄球菌肠毒素特征分析

Distribution of 20 enterotoxin and enterotoxin-like genes in Staphylococcus aureus strains isolated from healthy carriers in Beijing

目的了解健康从业人员携带金黄色葡萄球菌的肠毒素分布情况。方法收集北京市朝阳区健康从业人员鼻部携带金黄色葡萄球菌100株,对20种肠毒素和类肠毒素基因进行检测,并进行spa分型和多位点序列分型(MLST)分析。结果 100株金黄色葡萄球菌菌株中,64株菌(64%)携带1种或多种肠毒素基因。最常见的肠毒素基因分别为sea、seg、selm、seln、sell和seb。最常见的肠毒素类型为sea和selp,检出率均为10%。肠毒素sea主要分布在ST6(29.4%,5/17)和ST401(29.4%,5/17)型菌株。携带单一selp肠毒素基因的菌株50%均属于spa t796型。结论健康从业人员金黄色葡萄球菌肠毒素基因的高携带率可能成为食物中毒不可忽视的危险因素。

Objective To investigate the distribution of 20 enterotoxin （SE） or enterotoxin-like （SE1） genes in Staphylococcus （S.） aureus strains isolated from healthy carders. Methods A total of 100 S. aureus strains were collected from healthy carriers in Chaoyang district of Beijing to detect 20 SE and SE1 genes with PCR. Spa typing and multiple locus sequence typing （MLST） were performed. Results Among the 100 S. aureus strains, 64 carried one or more SE or SE1 genes. The most common genes were sea, seg, selm, seln, sell and seb. The most common enterotoxin were sea and selp, which accounted for 10% respectively. Enterotoxin sea was mainly distributed in ST6 （29.4%, 5/17 ） and ST401 strains （ 29.4% , 5/17 ）, while 50% of selp carrying strains belonged to spa t796. Conclusion The high carriage of enterotoxin in S. aureus strains isolated from healthy carriers might be a risk factor for food poisoning.