蝴蝶兰高效再生体系与遗传转化体系的建立

Establishment of Efficient Regeneration System and Genetic Transformation System of Phalaenopsis

为建立蝴蝶兰(Phalaenopsis amabilis)再生体系和遗传转化体系,对蝴蝶兰进行人工授粉,成熟后将胚播种于诱导培养基上诱导原球茎或不定芽。探讨外源调节剂的种类及浓度,并筛选卡那霉素浓度以及除菌剂的种类及浓度。结果表明:当6-BA浓度为5 mg·L^(^(-1)),NAA浓度为1 mg·L^(-1)时,原球茎的诱导效率最高。当6-BA浓度为1.5mg·L^(-1),同时添加0.5g·L^(-1)活性炭和40mL·L^(-1)椰汁时,壮苗效果最佳。卡那霉素的筛选浓度为4mg·L^(-1),除菌剂的种类为阿莫西林克拉维酸钾(7∶1),除菌浓度为100mg·L^(-1)。

In order to establish the regeneration system and genetic transformation system of Phalaenopsis ama- bilis,Phalaenopsis amabilis was used for artificial pollination, after maturation, the embryos were seeded on the induction medium to induce protocorm or adventitious buds. The type and concentration of exogenous reg- ulators were explored,and the concentration of kanamycin and the type and concentration of the fungicide were screened. The results showed that when the concentration of 6-BA was 5 mg·L-1 and NAA was 1 mg·L-1 ,the induction efficiency of protocorm was the highest. When the concentration of 6-BA 1.5 mg· L-1 ,while adding 0.5 g·L-1 activated carbon and 40 mL·L-1 coconut,the best effect of strong seedlings. The results showed that the concentration of kanamycin was 4 mg·L-1 ,the type of fungicide was amoxicillin clavulanate （7：1）,and the concentration was 100 mg·L-1.