肺炎支原体感染的两种不同实验室检测方法分析

Analysis of two different laboratory dignosis methods in detection of mycoplasma pneumoniae infection

目的通过对被动凝集法检测肺炎支原体(mycoplasma pneumoniae,MP)抗体与荧光定量PCR法检测肺炎支原体核酸的对比分析,探讨两种不同肺炎支原体感染实验室检测方法在临床诊断中的价值。方法选择我院儿科支气管肺炎病例848例,进行肺炎支原体抗体检测、肺炎支原体核酸检测,对两种方法的检测结果进行统计学分析。结果在出院确诊肺炎支原体感染的462例病例中,肺炎支原体MP核酸检测阳性占总检出肺炎支原体感染的55.63%,MP抗体检测阳性占总检出肺炎支原体感染的79.00%。二者结合检测阳性检测率为99.56%。两种方法男女阳性感染率差异无显著性;各年龄组检测阳性分布,90%的肺炎支原体感染阳性结果集中在1至9岁,95%阳性集中在1-12岁。结论两种方法检测肺炎支原体感染,都显示肺炎支原体感染在年龄上以1-9岁儿童高发,性别上无差异;在临床诊断上两种方法相互弥补,建议同时选择两种方法以提高临床的诊断率。

Objective To explore the clinical diagnosis value in two different clinical lab＇s diagnosis of mycoplasma pneumoniae through comparative analysis of detecting mycoplasma pneumoniae antibody by Passive Particle Agglutina- tion and detecting mycoplasma pneumoniae DNA by PCR Fluorescence Quantification. Methods Detect mycoplasma pneumoniae antibody and mycoplasma pneumoniae DNA in 848 cases in-patient children of bronchopneumonia and analysis the result by statistics method. Results Positive rate of mycoplasma pneumoniae antibody is 55.63% and mycoplasma pneumoniae DNA is 79.00 % in 462 cases those had been diagnosised infection of mycoplasma pneumoniae. Two methods together had detected 99.56% infection of mycoplasma pneumoniae. And the positive rates between two methods had no statistical significance difference in different sex. About 90% mycoplasma pneumoniae infection children＇s age were from 1 to 9 years old,and 95% of them were from 1 to 12 years old by two methods showing. Conclusion Two methods both showed infection of mycoplasma pneumoniae usually from 1 to 9 years old and having no sex difference; And detecting mycoplasma pneumoniae antibody by Passive Particle Agglutination and detecting mycoplasma pneumoniae DNA by PCR Fluorescence Quantification can make up eath other＇ s shortage, we suggest choicing two methods together in dignosis of infection of mycoplasma pneumoniae.