三维打印双相磷酸钙陶瓷支架在骨组织工程中的应用

The application of Biphasic calcium phosphates ceramic scaffold by 3D printing in bone tissue engineering

目的探索研究三维打印技术制备的由羟基磷灰石/β-磷酸三钙(HA/β-TCP)组成的双相磷酸钙陶瓷(BCP)支架在骨组织工程中的应用潜力,为其作为骨组织工程支架提供试验基础。方法通过三维打印技术制备质量比为3∶7的HA/β-TCP复合纳米支架,扫描电镜(SEM)观察支架形态。将支架与兔骨髓间充质干细胞共培养;通过CCK-8实验检测支架的生物相容性以及种子细胞在支架上的增殖分;并以相同材料制备的压模片进行比较。结果三维打印技术制备的双相磷酸钙陶瓷支架为三维均匀多孔隙结构,骨髓基质干细胞能够在此支架上很好的增殖,分化。结论三维打印制备双相磷酸钙陶瓷支架有较好的相容性,可促进细胞的分化,可作为细胞支架应用于骨组织工程中。

Objective To evaluated the biphasic calcium phosphates ceramic scaffold by 3D printing, which is consists of hydroxyapatite and β-tricalcium phosphate （HA/β-TCP）,and provide the experimental basis used for bone tissue engineering. Methods 3D printing technology was applied to prepare porous BCP scaffolds,and BCP cylinder was were prepared by mold materials. The composition ratio of BCP （% HA/% β -TCP） was 30/70. Scaffold was observed by scanning electron microscopy （SEM） . Rabbit bone mesenchymal stem cells were seeded on the scaffolds and cultured together. Results Scaffold-BMSCs were investigated with regard to cellular,spreading and proliferation through analyses of CCK-8. Conclusion The BCP scaffold had a good biocompatibility and may be a promising material in bone tissue engineering.