红霉素对烟草烟雾提取物作用下人巨噬细胞释放相关炎症因子的影响

The influence of erythromycin on the expressions of inflammatory cytokines in human macrophage cells stimulated by cigarette smoke extract

目的:研究红霉素(EM)对烟草烟雾提取物(CSE)作用下人巨噬细胞释放相关炎症因子的影响。方法:体外培养人类单核细胞系U937细胞,在细胞培养液中加入佛波酯(PMA)将其诱导分化为人巨噬细胞。将细胞分为3组:对照组、CSE组、EM+CSE组。CSE组用1%浓度CSE刺激24h,EM+CSE组用1mg/L EM预处理24h后,用1%浓度CSE刺激24h。用液相芯片技术检测各组细胞上清液中嗜酸细胞活化趋化因子(eotaxin)、白介素(IL)-17A、巨噬细胞炎症蛋白(MIP)-1β、单核细胞趋化蛋白(MCP)-1、IL-8、MIP-1α、IL-1β、IL-6含量。结果:CSE显著促进人巨噬细胞释放eotaxin、IL-17A、MIP-1β、MCP-1、IL-8、MIP-1α、IL-1β、IL-6(P<0.05),而EM能抑制CSE引起的上述炎症因子释放增加(P<0.05)。结论:EM可能通过抑制CSE刺激引起的人巨噬细胞释放eotaxin、IL-17A、MIP-1β、MCP-1、IL-8、MIP-1α、IL-1β、IL-6等细胞因子,抑制烟草烟雾暴露相关疾病进展。

Objective：To investigate the effect of erythromycin （EM） on the release of inflammatory cytokines in human macrophages stimulated by cigarette smoke extract （CSE）. Methods： The human monocytic cell line U937 cells were cultured in vitro, and differentiated into human macrophages by phorbol esters （PMA）. The cells were divided into 3 groups： control group, CSE group （cells were stimulated by 1% CSE）, and EM＋CSE group （cells were pretreated with 1 mg/L EM for 24 h and then stimulated by 1% CSE）. The Luminex LiquiChip was used to measure levels of the following cytokines： eotaxin, interleukin （IL）-17A, macrophage inflammatory protein （MIP）-1β, monocyte chemoattractant protein （MCP）-1, IL- 8, MIP-1, IL-1 and IL-6. Results： CSE significantly promoted the release of inflammatory cytokines （eotaxin, IL-17A, MIP-1β, MCP-1, IL-8, MIP-1α, IL-1β and IL-6） （P 〈0.05）, whereas pretreated with EM blocked the effect of CSE （ P 〈0.05）. Conclusion： EM could inhibit CSE-induced inflammatory cytokines （eotaxin, IL-17A, MIP-1 β, MCP-1, IL-8, MIP-1α, IL-1β and IL-6） release by human macrophages, which may prevent tobacco smoke exposure related diseases.