杨树MYB转录因子家族基因应答盐胁迫表达特性分析

Expression Analysis of Poplar MYB Transcription Factor Gene Family in Response to Salt Stress

MYB转录因子家族是植物中数量最多的转录因子家族之一,在植物次生代谢调节、信号转导和抗逆等生物过程起重要作用。根据MYB转录因子结构域组成差异可分4个亚家族:即1R-MYB(MYB-relaed)、R2R3-MYB、3R-MYB和4R-MYB。其中,R2R3-MYB亚家族数量最多,可进一步分为22个亚组;利用生物信息学分析杨树MYB转录因子蛋白序列的保守结构域、系统发生、基因组定位、氨基酸组成和理化性质等;参照拟南芥MYB转录因子功能,预测杨树MYB转录因子功能;基于84K杨转录组测序和RT-qPCR分析,从301个杨树MYB转录因子基因中筛选出69个应答盐胁迫基因(P≤0.05)。其中,上调表达基因32个,下调表达基因37个。该研究可为进一步研究杨树MYB家族基因功能提供参考依据。

MYB transcription factor family is one of the most important transcription factors in plants, and plays an important role in the process of plant secondary metabolism regulation, signal transduction and stress resistance. According to the difference of domain structure of MYB transcription factor, it can be divided into 4 subfamilies： 1R-MYB（MYB-relaed） , R2R3-MYB, 3R-MYB and 4R-MYB. Among them, the R2R3-MYB sub family with the largest number of members can be further divided into 22 subgroups. Using bioinformatics, the characterizations of conserved domain of Poplar MYB transcription factors, genomic location, amino acid composition and physicochemical properties were analyzed. According to the Arabidopsis MYB transcription factor, the functions of Poplar MYB transcription factor were predicted. Based on the transcriptome sequencing and RT-qPCR, 69 salt-stress responding genes （P ≤0.05） were screened from 301 MYB genes, including 32 up-regulated genes and 37 down regulated genes. The study could provide a reference for further study on the function of MYB family genes in Poplar.