超声辅助低共熔溶剂提取沙棘籽粕多酚的工艺优化

Process Optimization of Ultrasonic Assisted Deep Eutectic Solvents(DESs) Extraction of Polyphenols from Hippophae rhamnoides Seed Meal

以一系列低共熔溶剂为提取剂,采用超声波辅助法从沙棘籽粕中提取多酚。在单因素试验结果基础上,利用Box-Behnken实验设计,运用响应面分析法对影响沙棘籽粕多酚得率的主要因素(超声功率、超声时间、超声温度)进行优化。结果表明,沙棘籽粕多酚最佳提取工艺条件为:以含水量为30%的氯化胆碱—草酸低共熔溶剂为最佳提取剂,液料比为14∶1,超声功率420 W,超声时间56 min,超声温度44℃。在此条件下,多酚得率为3.31±0.008%。对比试验发现:氯化胆碱—草酸低共熔溶剂对沙棘籽粕多酚的得率明显优于传统溶剂;与热回流提取相比,超声提取法具有明显的优势。

A range of deep eutectic solvents （DESs） as the solvent were used for extraction polyphenols with ultrasonic-assisted extraction methods from sea buckthom. According to the single-parameter study, experiment factors （ultrasonic power, ultrasonic time, ultrasonic temperature） remarkably affected the yield of polyphenols were selected and Box-Behnken experimental design was investigated to be optimized by response surface methodology. The results indicated that with 30% water in Choline chloride-Oxalic acid as the suitable solvent and liquid to material ratio of 14：1, the optimal conditions of ultrasonic extraction were as follows： ultrasonic power of 420 W, ultrasonic time of 56 min, and ultrasonic temperature of 44℃. Under these conditions, the yield of polyphenols was （3.31 ± 0.08 ）%. Comparison experiment indicated that the extraction efficiency of polyphenols from sea buckthorn seed meal by DES was superior to the traditional solvent. Compared with the heat refluxing extraction method, DESs extraction method was a extraction technology with high efficiency.