高效液相色谱法测定异叶爬山虎中异槲皮苷的含量

Determination of the content of isoquercitrin in Parthenocissus heterophylla(BI.) by HPLC

目的建立高效液相色谱法(HPLC)测定异叶爬山虎中异槲皮苷含量的方法,并测定广西不同地区的异叶爬山虎中异槲皮苷的含量。方法采用Inersil-C_(18)色谱柱(4.6 mm×25 mm,5μm),流动相为甲醇-0.05%磷酸溶液(46∶54),流速1 m L·min^(-1),检测波长258 nm,柱温25℃,进样量10μL,分析时间32 min。结果异槲皮苷的进样量在20~200μg范围内,与峰面积呈良好的线性关系,相关系数r=0.999 9(n=6);异槲皮苷的平均回收率为98.53%(n=6),RSD为0.79%。结论该法操作简便,重复性好,可用于测定异叶爬山虎中异槲皮苷含量。

Objective To establish a method for the determination of Isoquercitrin in EAE extract of Parthenocissus heterophylla（ BI.）Merr. Methods The Component was separated on a Inersil C_（18） column（ 4. 6 mm × 25 mm,5 μm） with a flow-rate of 1 m L·min-1 at25 ℃. The mobile phase consisted a mixture of methanol and 0. 05% phos phoric acid（ 46∶ 54）. The total analysis time was 32 min.The detector was DAD and the detection wavelength was 258 nm. Results The linear range of Isoquercitrin was 20 ～ 200 μg,（ r =0. 999 9）. The average recovery was 98. 53%,RSD was 0. 79%（ n = 6）. Conclusion The method is simple and accurate,and can be used for the determination of content of Isoquercitrin in the EAE extract of Parthenocissus heterophylla（ BI.） Merr.