免疫组织化学检测P16、Ki67在宫颈鳞状上皮内病变中的应用

The application and significance of immunohistochemical detection of P16 and Ki67 in cervical squamous intraepithelial lesions

目的分析免疫组化P16、Ki67在宫颈鳞状上皮内病变中的应用及意义。方法收集经活检或手术切除宫颈组织标本,其中宫颈鳞状上皮内病变80例将其作为病例组,此外将30例非宫颈病变手术切除正常宫颈组织标本作为对照组。采用S-P免疫组化方法对所有标本进行P16、Ki67表达的检测,并分析其表达及意义。结果 P16阳性率依次由对照组、CINⅠ、CINⅡ、CINⅢ组呈上升趋势,对照组与病例组两两比较差异有统计学意义(P<0.05);对照组Ki67阳性率明显低于病例组CINⅠ、CINⅡ、CINⅢ,差异有统计学意义(P<0.05),病例组内比较差异无统计学意义(P>0.05);P16、Ki67的关联性比较差异无统计学意义(P>0.05)。结论 Ki67和P16的表达情况可有效甄别子宫颈良性反应病变情况,但二指标的关联性不强,提示其对病变的指示方向并非相同,因此,对于Ki67和P16的表达情况进行联合检测,可以更为公允的帮助相关人员鉴定子宫颈病变情况,值得在临床中进一步推广。

Objective To analyze the application and significance of Immunohistochemical P16 and Ki67 in cervical squamous intraepithelial lesions. Methods Eighty cases of cervical squamous intraepithelial lesions collected from menstruation biopsy or the cervical tissue of surgical removal samples were selected as the case group. 30 cases specimens of normal cervical tissues were resected by surgery of non-cervical lesions were taken as the control group. S-P immunohistochemical method was taken to detect p16,Ki67 in all the specimens from which the expressions were analyzed. Results The positive rate of P16 were elevated in the control group,CIN Ⅰ,CINⅡ,CINⅢ group,and there was significant difference between the control group and case group（ P〈0. 05）. The positive rate of Ki67 in the control group was significantly lower than those in the CINⅠ,CINⅡ,CINⅢ groups,and there existed statistically significant differences（ P〈0. 05）. The positive expression of P16 and Ki67 was no statistical significance（ P〈0. 05） in the case group.Conclusion The expression of Ki67 and P16 can effectively identify the cervical benign lesions. However the correlation which is not strong means that the tip on the direction of pathological changes is not the same. Therefore,the associated detection of Ki67 with P16 show promise to identification of cervical lesions,which is worth popularizing in the clinic.