CCL21对胃癌SGC-7901细胞发生上皮间质转化的影响

Effects of CCL21 on inducing epithelial-mesenchymal transition in gastric cancer cells SGC-7901

目的研究CCL21能否参与诱导胃癌SGC-7901细胞发生上皮间质转化。方法取不同浓度(0、50、100、150μg·L^(-1))的CCL21作用胃癌SGC-7901细胞,划痕试验检测该细胞侵袭能力。镜下观察该细胞形态的变化。Western blot分别检测Ecadherin、N-cadherin、Vimentin、MMP-9的蛋白表达。结果 150μg·L-1浓度的CCL21作用胃癌SGC-7901细胞后,较其它浓度侵袭能力强,同时使胃癌SGC-7901细胞的胞体逐渐变为长梭状,部分可见伪足生成。E-cadherin蛋白表达下降(P<0.05),Ncadherin、Vimentin、MMP-9蛋白表达升高(P<0.05)。结论 CCL21可能参与诱导胃癌SGC-7901细胞发生上皮间质转化。

Objective To investigate whether CCL21 can induce epithelial-mesenchymal transition in gastric cancer cells SGC-7901.Methods SGC-7901 cells were induced by CCL21 in different concentration（ 0,50,100,150 μg·L-1） and then the ability of invasion and metastasis was detected by scratch testing. Morphological changes in forms were observed. The protein expressions of E-cadherin,N-cadherin,Vimentin,MMP-9 were detected by Western blot. Results After being induced by CCL21 in 150 μg·L-1,SGC-7901 cells achieved faster ability of invasion and metastasis. SGC-7901 cells formed into a long spindle and pseudopod could be seen. The expressions of N-cadherin,Vimentin,and MMP-9 rose（ P〈0. 05） while that of E-cadherin obviously descended（ P〈0. 05）. Conclusions CCL21 may induce epithelial-mesenchymal transition in gastric cancer cell and promote the invasion and metastasis.