1-甲基乙内酰脲抑制家兔生长激素的分泌

1-methylhydantoin inhibits secretion of growth hormone in rabbits

目的探索1-甲基乙内酰脲(1-methylhydantoin,MH)抑制家兔生长激素(growth hormone,GH)基础分泌以及拮抗生长激素释放激素促分泌的作用。方法将36只家兔按给药种类不同,随机分为生理盐水组(A组)、MH组(B组)、奥曲肽组(C组)、生长激素释放激素组(D组)、生长激素释放激素+MH组(E组)和生长激素释放激素+奥曲肽组(F组),每组6只。各组家兔均在注射药物之前和之后5、15、30、45、60 min各采血1.0 mL,利用酶联免疫吸附测定试剂盒检测血清GH水平,同时,密切观察各组家兔在注射相应药物后的行为活动情况(1周)。结果 1A组家兔各个时间点的GH水平无差异(P>0.05);2与A组相比,B组和C组家兔GH水平明显降低(P<0.05),而D组家兔GH水平明显升高(P<0.05);3与D组相比,E组和F组家兔GH水平明显降低(P<0.05);4各组实验家兔在静脉注射相应药物后,1周之内未观察到明显的毒副反应。结论 1 MH能抑制家兔GH的基础分泌;2MH能拮抗生长激素释放激素的促分泌作用。

Aim Toexplorewhether1-methylhydan-toin（MH）could inhibit the basal secretion of growth hormone （GH ） and suppress the promoting effect of growth hormone-releasing hormone （GHRH ） in rab-bits.Methods Thirty-sixrabbitswererandomlydi-vided into six experimental groups according to the kind of dosing drugs,namely normal saline group（A）, MH group （B ）,octreotide group （C ）,GHRH group （D）,GHRH ＋MH group（E）,GHRH ＋octreotide group（F）,with 6 rabbits in each group.Blood was sampled （1. 0 mL each time）from each rabbit before injecting drugs and 5,15,30,45,60 min after drug administration.Clotting spontaneously,rabbits blood samples were centrifugated for 20 minutes at approxi-mately 1000 ×g and the supernatant was collected. Serum GH concentrations were determined by enzyme linked immunosorbent assay kit（ELISA Kit）.Mean-while,the behavior of rabbits in each group after injec-tingdrugswascloselyobserved.Results TheGH level of rabbits in group A at each time point had no significant differences（P〉0. 05 ）.Group B and group C rabbit GH levels were significantly lower than those of group A （P〈0. 05 ）,while GH levels in group D were obviously higher than those of group A （P 〈0. 05 ）.Compared with group D,rabbit GH levels in group E and group F decreased markedly（P〈0. 05 ）. No obvious toxic and side effects had been observed within one week after the experimental rabbits were ad-ministered corresponding drugs by intravenous injec-tion.Conclusions 1-methylhydantoincouldinhibit the basal secretion of GH in rabbits.1-methylhydan-toin could suppress the promoting effect of GHRH in rabbits.