Wilms瘤基因在白血病、多发性骨髓瘤和淋巴瘤中的表达研究

Expression of WT1 gene in leukemia,multiple myeloma and lymphoma

目的探讨Wilms瘤基因(WT1)在白血病、淋巴瘤及多发性骨髓瘤患者中的表达差异及其临床意义。方法选取77例白血病、29例多发性骨髓瘤和30例淋巴瘤患者进行研究,采用荧光定量PCR技术检测三组骨髓中WT1基因的表达水平并进行比较分析。结果白血病患者的WT1基因水平(0.105±0.019)显著的高于多发性骨髓瘤患者(0.027±0.008)和淋巴瘤患者(0.026±0.010)且差异有统计学意义(P<0.05),多发性骨髓瘤患者和淋巴瘤患者的WT1基因表达水平差异无统计学意义(P>0.05)。治疗后,白血病患者的WT1基因水平(0.062±0.016)显著的低于初诊的白血病患者(0.157±0.012)且差异有统计学意义(P<0.05),治疗后的多发性骨髓瘤患者和淋巴瘤患者与初诊的多发性骨髓瘤患者和淋巴瘤患者的WT1基因表达水平差异无统计学意义(P>0.05)。结论 WT1在白血病患者表达水平较淋巴瘤、多发性骨髓瘤患者呈显著的升高,同时治疗后白血病患者的WT1基因表达水平较初诊白血病患者呈显著降低。

Objective To investigate the expression and clinical significance of Wilms tumor gene（WT1）in patients with leukemia,lymphoma and multiple myeloma.Methods Seventy-seven leukemia patients,29 multiple myeloma patients and 30 malignant lymphoma patients were selected as the research objects from Affiliated Hospital of Hubei Polytechnic University.The expression levels of WT1 gene in three groups of bone marrow were detected by fluorescence quantitative PCR technique,and three groups＇ expression levels of WT1 gene were compared and analyzed.Results The level of WT1 gene of leukemia patients（0.105 ± 0.019）was significantly higher than those of multiple myeloma patients（0.027 ± 0.008）and lymphoma patients（0.026 ± 0.010）（P〈0.05）.The expression levels of WT1 gene of multiple myeloma patients and lymphoma patients were not statistically significant（P〈0.05）.The expression level of WT1 gene of leukemia patients（0.062 ± 0.016）was significantly lower than that of newly diagnosed leukemia patients（0.157 ±0.012）and the difference was statistically significant（P〈0.05）.Differences were not significant between multiple myeloma patients and lymphoma patients after treatment and those newly diagnosed in the level of WT1 gene（P〈0.05）.Conclusions WT1 expression levels were significantly higher in patients with leukemia than those with lymphoma and multiple myeloma,and the level of WT1 gene expression in patients with leukemia after treatment was significantly lower than the newly diagnosed leukemia patients.