意大利蜜蜂血淋巴HP19蛋白的表达及功能

Expression of the 19 k Da Hemolymph Protein in honeybee Apis mellifera ligustica

昆虫血淋巴蛋白HP19主要参与蜕皮激素调控昆虫的变态及发育进程,蜂群内的工蜂、蜂王和雄蜂具有不同的变态和发育历期,为探究hp19在调控意大利蜜蜂Apis mellifera ligustica三型蜂变态发育中的作用,本研究利用实时荧光定量PCR技术对hp19在不同发育时期的工蜂、雄蜂和蜂王体内的转录水平进行检测。结果表明,该基因在工蜂和雄蜂3龄幼虫体内表达量分别为参照基因的104和105倍,在5龄幼虫体内的表达则分别降为参照的10和102倍,蛹期表达量又都显著提高。hp19在蜂王整个幼虫期的表达保持在参照的102倍,封盖后逐渐上升,至化蛹前达到参照的105倍。在成年工蜂体内的表达为参照的104倍,而在新羽化蜂王和雄蜂体内表达水平为参照的107倍,但在性成熟的产卵蜂王和雄蜂体内的表达显著降低至参照的104倍。hp19在三型蜂体内这种不同的表达模式说明其不仅与三型蜂差异的变态发育历期有关,还可能与雄蜂和蜂王的生殖有关,具有多样的生物学功能。

Insect 19 kDa haemolymph protein mainly participate in regulation the metamorphosis development. There are three type of bees with different development duration in honeybee colony. To explore the role of hpl9 in regulation the metamorphosis development of honeybee Apis mellifera ligustica, its expression in different developing stage of worker, queen and drone were determined by using Real-time PCR technology. The results showed that the expression of hp／9 were 104 and 10 5 times of reference in 3rd instar larvae of worker and drone respectively, decreased to 10 and 102 times of reference in 5 rd instar larvae of worker and drone respectively, and both increased significantly in pupae stage. However, hp／9 was 102 times of reference expressed in whole larvae stage of q u een , gradually increased in caped larvae, and reached to 105 times of reference in late pupae stage. While in adult b ees , hp／9 was 104 times of reference expressed in worker bee, but significantly high expressed in new emerged queen or drone with an expression of 107 times of reference, then decreased to 104 times of reference in old drone or egg laying queen. Such a different expression of hp／9 suggest it not only related to the difference in metamorphosis development of three castes of honeybee, but also be associated with the reproductive of drone and queen,and thus might have multiple biological function.