摘要
采用正相硅胶、MCI、反相RP-18和半制备液相等色谱技术,对柏科翠柏属植物翠柏(Calocedrus macrolepis)中的二萜类成分及其抗炎活性进行了研究。结果表明:从中共分离得到8个二萜类化合物,分别鉴定为8-hydroxy-labda-13(16),14-dien-19-yl trans-coumarate(1)、trans-communal(2)、trans-communic acid(3)、pinusolidic acid(4)、isocupressic acid(5)、fokihodgin F(6)、acetylisocupressic acid(7)、15,16-dihydroxy-labda-8(17),13(E)-dien-19-oic acid(8)。化合物1~8均为首次从该植物中分离得到。化合物7对LPS诱导的RAW264.7巨噬细胞释放NO具有显著的抑制作用,其IC50值为9.31μmol·L^(-1)。
To study the diterpenoids from Calocedrus macrolepis and their anti-inflammatory activities, the constituents were separated by silica gel, MCI, RP-18, semi-preparative HPLC and eight compounds were isolated. Their structures were characterized as： 8-hydroxy-labda-13（16）,14-dien-19-yl trans-coumarate（1）, trans-communal（2）, trans-communic acid（3）, pinusolidic acid（4）, isocupressic acid（5）, fokihodgin F（6）, acetylisocupressic acid（7）, 15,16-dihydroxy-labda-8（17）,13（E）-dien-19-oic acid（8）. Compounds 1-8 were obtained from this plant for the first time. Among them, Compound 7 exhibited potent inhibitory effect on NO production in LPS-induced macrophages with IC50 values of 9.31 μmol·L^-1.
作者
丁林芬
吴兴德
王双燕
涂文超
潘争红
郭亚东
宋流东
DING Lin-Fen WU Xing-De WANG Shuang-Yan TU Wen-Chao PAN Zheng-Hong GUO Ya-Dong SONG Liu-Dong(School of Pharmaceutial Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China State Key Laboratroy of Phytochemistry and Plant Resources in Western China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China College of Rehabilitation and Nursing, Yunnan Business Management College, Kunming 650106, China Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, Guangxi, China)
出处
《广西植物》
CSCD
北大核心
2017年第5期642-646,633,共6页
Guihaia
基金
云南省天然药物药理重点实验室开放研究基金(2015G004)
关键词
柏科
翠柏属
翠柏
二萜
成分
抗炎活性
Cupressaceae, Calocedrus, C. macrolepis, diterpenoids, constituents, anti-inflammatory activity
