摘要
目的探讨非小细胞肺癌(NSCLC)组织中微小RNA(miRNA)-449a/b/c的表达水平、临床意义及与预后的关系。方法收集2013年1月至2015年12月收治的82例手术切除的NSCLC组织及69例癌旁组织标本,采用荧光实时定量PCR(QPCR)法检测以上组织中miR-449a/b/c的表达并比较3者在NSCLC组织及癌旁组织中的分布差异,Pearson相关分析NSCLC组织中miR-449a、miR-449b和miR-449c表达的相关性,分析NSCLC组织中miR-449a/b/c表达与临床病理特征(性别、年龄、肿瘤大小、TNM分期、组织学类型和淋巴结转移)的关系,同时根据随访数据比较不同miR-449a/b/c表达患者的预后情况。结果 QPCR结果显示,NSCLC组织中miR-449a、miR-449b和miR-449c的平均表达量依次为0.210±0.028、0.359±0.031和0.133±0.020,均低于癌旁组织,差异有统计学意义(P<0.05);NSCLC组织中miR-449a、miR-449b和miR-449c的表达均呈正相关,相关系数r_(miR-449a/b)、r_(miR-449a/c)和r_(miR-449b/c)分别为0.246、0.390和0.331(P<0.05);NSCLC组织中miR-449a/b/c表达均与TNM分期有关,miR-449a和miR-449c表达与肿瘤大小有关。全组中位总生存期(OS)为12.4个月,其中miR-449a低表达组和高表达组的中位OS分别为11.2个月和13.5个月(P>0.05),miR-449b低表达组和高表达组的中位OS分别为9.6个月和14.2个月(P<0.05),miR-449c低表达组和高表达组的中位OS分别为11.7个月和13.7个月(P>0.05)。结论miR-449a/b/c在NSCLC组织中表达降低,且均与TNM分期有关,miR-449b表达与预后有关,可能与NSCLC发生、发展有关,对NSCLC诊断及病情评估有一定价值。
Objective To investigate the expression and clinical significance of miR-449a/b/c in non-small cell lung cancer（ NSCLC） as well as their expressions with prognosis. Methods We collected 82 cases of surgically resected NSCLC tissues and 69 cases of adjacent normal tissue（ 〉 5 cm away from cancer tissue） from January 2013 to December 2015. The expressions of miR-449a/b/c of above tissues were detected by using real-time fluorescence quantitative PCR（ QPCR）. The differences of distribution of miR-449a/b/c expressions in NSCLC and adjacent normal tissues were compared. Pearson correlation analysis of miR-449 a,miR-449 b and miR-449 c in NSCLC tissues was undergone. The relationship of miR-449a/b/c expressions in NSCLC with clinical pathological parameters（ gender,age,tumor size,TNM stage,histological type and lymph node metastasis） were analyzed. According to the followup data,the prognosis of different expressions of miR-449a/b/c was compared. Results QPCR results showed that in NSCLC tissues,the avarage expressions of miR-449 a,miR-449 b and miR-449 c were 0. 210 ± 0. 028,0. 359 ± 0. 031 and 0. 133 ± 0. 020,lower than those in adjacent normal tissues,and the differences were statistically significant（ P 〈 0. 05）. The expressions of miR-449 a,miR-449 b and miR-449 c were positively correlated in NSCLC and correlation coefficients for miR-449a/b,miR-449a/c and miR-449b/c were 0. 246,0. 390 and 0. 331（ P 〈 0. 05）. The miR-449a/b/c levels of NSCLC tissues were significantly associated with TNM stage,and miR-449 a and miR-449 c were related to tumor size. All patients were followed up,and the median overall survival（ OS） was 12. 4months. The median OS for miR-449 a low expression group and high expression group were 11. 2 and 13. 5 months（ P 〉 0. 05）. The median OS for miR-449 b low expression group and high expression group were 9. 6 and 14. 2 months（ P 〈 0. 05）. The median OS for miR-449 c low expression group and high expression group were 11. 7 and 13. 7 months（ P 〉 0. 05）. Conclusion The expression of miR-449a/b/c in NSCLC tissues was decreased,and all of them were related to TNM stage. The expression of miR-449 b was related to prognosis,which may be related to the occurrence and development of NSCLC. It has some value in the diagnosis and evaluation of NSCLC.
出处
《临床肿瘤学杂志》
CAS
2017年第4期303-308,共6页
Chinese Clinical Oncology
