微小RNA-100与微小RNA-143在膀胱癌患者血清的表达及临床意义

Value of combined detection of the level of microRNA - 100 and microRNA - 143 in serum of blad. der cancer

目的探讨联合检测血清微小RNA（miRNA，miR）-100与miR-143水平对膀胱癌的诊断价值。方法应用实时定量反转录聚合酶链反应（RT-qPCR）检测112例膀胱癌患者和100例健康受试者血清中miR-100与miR-143的水平，并应用受试者工作特征（ROC）曲线计算miR-100与miR-143的临界值，并根据此临界值单独或联合检测血清中miR-100与miR-143水平，观察其诊断膀胱癌的灵敏度与特异度。结果血清miR-100相对表达水平在NMIBC组（0.213±0.087）和MIBC组（0.175±0.101）分别显著低于对照组（1.086±0.493；χ2=7.381，P=0.026；χ2=12.322，P=0.011）；血清miR-143相对表达水平在NMIBC组（0.193±0.075）和MIBC组（0.011±0.003）分别显著低于对照组（0.992±0.334；χ2=9.557，P= 0.019；χ2=16.926，P=0.008）；MIBC组血清miR-143相对表达水平（0.011±0.003）显著低于NMIBC组（0.193±0.075；χ2=4.346，P=0.033）。存在淋巴结转移的膀胱癌患者血清中miR-100与miR-143的相对表达水平（0.231±0.027、0.224±0.117）分别显著高于无淋巴结转移的膀胱癌患者（0.144±0.065、0.162±0.044；χ2=5.861，P=0.028；χ2=3.793，P=0.031）；血清miR-100与miR-143联合检测对诊断膀胱癌的灵敏度与特异度分别为89.73%与88.91%。结论膀胱癌患者血清中miR-100与miR-143水平显著降低；血清miR-100与miR-143联合检测有助于诊断膀胱癌。

ObjectiveTo observe the value of combined detection of the level of microRNA （miRNA, miR）-100 and miR-143 in serum of bladder cancer.MethodsSerum of 112 patients with bladder cancer and 100 cases of healthy people was collected, and real-time reverse transcriptase-polymerase chain reaction（RT-qPCR） was used to detect the level of miR-100 and miR-143. The receiver operating characteristic （ROC） curve was used to calculate the cut-off value of miR-100 and miR-143, and the value of combined detection of the level of miR-100 and miR-143 in serum of bladder cancer was assessed.ResultsThe relative expression levels of miR-100 in serum in NMIBC group （0.213±0.087） and muscle invasive bladder cancer （MIBC） group （0.175±0.101） were significantly lower than the control group （1.086±0.493; χ2=7.381, P=0.026; χ2=12.322, P=0.011）; the relative expression levels of miR-143 in serum in NMIBC group （0.193±0.075） and MIBC group （0.011±0.003） were significantly lower than the control group （0.992±0.334; χ2=4.346, P= 0.033）; the relative expression levels of miR-143 in serum in MIBC group（0.011±0.003） was significantly lower than the NMIBC group （0.193±0.075; χ2=4.346, P=0.033）. The relative expression levels of miR-100 and miR-143 （0.231±0.027, 0.224±0.117） were significantly higher than those without lymph node metastasis in patients with bladder cancer （0.144±0.065, 0.162±0.044; χ2=5.861, P=0.028; χ2=3.793, P=0.031）. The sensitivity and specificity of combined detection of miR-100 with miR-143 in the diagnosis of bladder cancer were 89.73% and 88.91% respectively.ConclusionLevels of miR-100 and miR-143 in serum of bladder cancer patients are reduced significantly. There is a high diagnostic value of combed detection of miR-100 with miR-143 to bladder cancer.