注射异丙肾上腺素建立大鼠心肌肥厚模型

Rat Model of Isoproterenol-induced Cardiac Hypertrophy

目的采用异丙肾上腺素诱导心肌肥厚,建立大鼠模型,并研究该动物模型的基本特性。方法大鼠背部皮下注射剂量为5 mg/kg的异丙肾上腺素,每日1次,连续注射14 d。结果模型组大鼠的全心重/体质量,左心室重/体质量均明显增加。模型组大鼠血清中羟脯氨酸(HYP)含量显著增加。模型组大鼠心肌组织中总超氧化物歧化酶(SOD)含量与对照组相比显著降低,而丙二醛(MDA)含量明显升高。结论皮下注射异丙肾上腺素14 d,可成功诱导大鼠心肌肥厚模型,为深入研究心肌肥厚的确切机制奠定基础。

Objective To establish a rat model of cardiac hypertrophy induced by isoproterenol （ISO）, and to study its basic characteristics. Methods Cardiac hypearophy was induced in rats with ISO. The model rats received subcutaneous injections of 5 mg/kg ISO every day for 14 days. Results The heart weight/body weight and left ventricular weight/body weight ratios in model rats were significantly increased. The serum hydroxyproline level was significantly increased, the superoxide dismutase level was significantly decreased, and the malondialdehyde level was sig- nificandy increased in model rats. Conclusion The rat model of cardiac hypertrophy is successfully created by subcutaneous injection of ISO for 14 days. This model can be used in study of the mechanism of cardiac hypertrophy.