瓶装水和桶装水中壬基酚含量调查

Study of nonylphenol content in bottled water and barrelled water

目的了解壬基酚(NP)在瓶装水和桶装水中的含量,为水的健康饮用提供数据支持。方法(1)采样及样本量:根据2016年瓶装水的销售数据,从排名前10的品牌中随机选取5个品牌,桶装水在遵义市办公单位选取常喝的3个品牌,每个品牌取5份样品测定。(2)样品处理:将1 000 m L样品用30、30、20 m L二氯甲烷萃取3次,加入甲醇0.5 m L。(3)样品测定:采用高效液相色谱进行测定。色谱条件为色谱柱:Elipse C18;流动相:甲醇-三蒸水(体积比为80∶20);柱温:30℃;流速:1 m L/min;波长:225 nm;检测器:VWD。结果 5个品牌的瓶装水中均检测出NP,检出率为100.0%,其含量为4.29~6.05μg/L。未加热的桶装水中NP的含量为3.21~4.24μg/L,加热后(70℃)桶装水中NP的含量为2.88~4.33μg/L。经ANOVA分析,各品牌瓶装水中NP的含量比较,差异有统计学意义(P<0.05);经配对t检验发现,加热前后桶装水中NP含量比较,差异无统计学意义(P>0.05)。结论市售瓶装水和桶装水中均检出NP,加热对NP含量影响不大,其含量虽低于美国环保局规定的饮用水标准6.60μg/L,但长期饮用对健康的危害值得重视。

Objective To understand the nonylphenol （NP） content in bottled water and barreled water,and to provide data support for healthy drinking. Methods （1）Sampling and sample size：according to the sales data for bottled water in 2016,5 brands from the top 10 were selected randomly,and 3 brands of barreled water which Zunyi city office units often drunk were selected, each brand took 5 samples to measure. （2）Sample handling： 1 000 mL water sample was extracted three times with 30, 30 and 20 mL earrene, and dissolved into 0.5 mL methanol. （3）Sample determination：the samples were measured by high performance liquid chromatographic. The chromatographic conditions ：chromatographic column ：Elipse C18;mobile phase ：methanol-three distilled water （volume ratio 80：20 ） ; column temperature ： 30 ℃ ; flow rate ： 1 mL/min ; wave length ： 225 nm ; detector： VWD. Results NP was detected in all 5 brands of bottled water,the relevance ratio of NP is 100.0% ,and the concentration was 4.29- 6.05 μg/L. The content of NP in barreled water which was not heated was 3.21-4.24 μg/L, after heating （70 ℃ ） the content of NP in barreled water was 2.88-4.33 μg/L. Analysed by ANOVA ,the difference of NP concentration among different brands of bottled water was statistically significant （P〈0.05）. The results of paired-samples t test showed no significant difference in the content of NP in barreled water before and after heating （P〉0.05） . Conclusion NP was detected in bottled water and barreled water. Heating has little effect on NP concentration. The concentrations are lower than the drinking water standard （6.6 μg/L） which is established by US Environmental Protection Agency, the impact to health is worth attention for long-term drinking.