白酒丢糟的多菌种混菌固态发酵制备有机肥

Optimization on Process Condition in Solid-state Fermentation of Vinasse for Organic Fertilizer

以白酒丢糟为主要原料,4种芽孢杆菌、2种霉菌与酿酒酵母为混菌发酵菌株,进行多菌种混菌固态发酵,以发酵产物的腐殖质、全氮、全磷、全钾含量为指标,通过单因素实验和响应面法分析探讨酒糟固态发酵制备有机肥在初始含水量、酒糟量和初始pH的最佳工艺条件.经验证性实验可知预测值与实际值相符,得到最佳工艺条件:初始含水量为60.54%、酒糟量为52.06%、初始pH为7.03,接种量为3%~5%,发酵时间为6 d,发酵产物中腐殖质含量达到17.50%,种子发芽指数GI值达到93.2%,E4/E6值为2.19.

Small experiment was carried out at room temperature for the production ot orgamc fertilizer using the grains of liquor as the carrier. In this experiment, wheat bran and distillers grains were used as substrates, 4 kinds of bacillus and 2 kinds of fungi and yeast were used as the strains for fermentation. This experiment explored the optimum conditions of the solid-state fermentation for organic fertilizer through the single factor tests and response surface method according the content of humus, nitrogen, phosphorus and potassium in the fermented product. The conditions included mainly the initial water content, vinasse content and the initial pH. According the verification test, the actual value was consistent with the estimated value, and the optimum conditions were con- firmed. The optimum conditions were that initial water content was 60. 54%, content of vinasse was 52. 06% , the initial pH was 7.03, the initial inoculum was between 3% and 5%, the duration of fermentation was 6 days, and the finally the humus content in the fermented product was 17.50%, germination index, GI was 93.2% , E4/E6 was 2. 19.