三维适形放射治疗联合化疗对食管癌患者外周血Treg的影响

Influence of three dimensional conformal radiation therapy combined with chemotherapy on Treg in peripheral blood of esophagus carcinoma patients

目的观察三维适形放射治疗(3D-CRT)联合化疗对食管癌患者外周血CD4+Foxp+调节性T细胞(Treg)的比例及转化生长因子β1(TGF-β1)水平的影响,探讨3D-CRT联合化疗治疗食管癌的内在机制。方法应用流式细胞术检测87例术后食管癌患者和30名健康者Treg比例,酶联免疫吸附试验(ELISA)检测血清TGF-β1水平,分析3D-CRT联合化疗患者、3D-CRT患者及健康者外周血Treg比例和TGF-β1水平差异。结果放化疗后,3D-CRT联合化疗组患者外周血单个核细胞(PBMC)中Treg占CD4^+T细胞的比例及血清TGF-β1水平较3D-CRT组显著下降(P<0.05)。结论 3D-CRT联合化疗能有效下调食管癌患者外周血Treg比例和血清TGF-β1水平,对食管癌治疗具有重要意义。

Objective To investigate the influence of three dimensional conformal radiation therapy （3D-CRT） combined with chemotherapy on CD4＋ Foxp＋ regulatory T cell （Treg） proportion and transforming growth factor-beta 1 （TGF-β1） level in peripheral blood of esophagus carcinoma patients,and to investigate the internal influence mechanism of 3D-CRT combined with chemotherapy for the treatment of esophagus carcinoma. Methodse Flow cytometry was used to determine Treg proportion in 87 esophagus carcinoma patients and 30 healthy subjects. Serum TGF-β1 level was determined by enzyme-linked immunosorbent assay （ELISA）. The difference of Treg proportion and TGF-β1 level was analyzed among patients undergoing 3D-CRT combined with chemotherapy,patients with single 3D-CRT and healthy subjects. Results Compared to single 3D-CRT group,the Treg proportion and TGF-β1 level in peripheral blood decreased in 3D-CRT combined with chemotherapy group after patients undergoing radiochemotherapy （P〈0.05）. Conclusions The 3D-CRT combined with chemotherapy could reduce Treg proportion and TGF-β1 level in peripheral blood of esophagus carcinoma patients,and plays a role for the treatment of esophagus carcinoma.