新型二硫化钼荧光传感器检测乳腺癌相关miRNA

MoS_2-based fluorescence sensor for the detection of breast cancer-related microRNA

目的建立一种快速简便、高敏感性、高特异性的基于新型纳米材料二硫化钼的荧光传感器用于乳腺癌相关微小RNA(miRNA)检测的方法。方法在荧光标记的DNA探针体系中加入新型纳米材料二硫化钼构建一种荧光传感器,然后在荧光传感器中分别加入非互补miRNA、单碱基错配的miRNA、不同浓度的互补miRNA与荧光标记的DNA探针进行杂交,通过检测杂交前后荧光信号的变化来检测miRNA。结果检测只需40 min即可完成,对互补miRNA的检出限可达pmol/L级,且能够将互补、非互补、单碱基错配的miRNA区分开来。结论该新型的纳米材料二硫化钼荧光传感器分析速度快、敏感性和特异性高,建立的检测方法可应用于乳腺癌相关miRNA的快速检测,是一种非常有潜力的检测方法。

Objective To establish a fast,convenient,sensitive and specific fluorescence sensor based on novel nanomaterial MoS2 to detect breast cancer-related microRNA（miRNA）. Methodse The fluorescence sensor was fabricated by mixing novel nanomaterial MoS2 with fluorescent dye-labeled DNA probe. Noncomplementary miRNA,one-base mismatched miRNA and different concentrations of complementary miRNA were introduced to fluorescence sensor for hybridization with DNA probe. The miRNA was detected through monitoring fluorescence signal change before and after DNA probe-miRNA hybridization. Results The pmol/L level of complementary miRNA could be detected within 40 min,and fluorescence sensor could discriminate complementary miRNA from noncomplementary miRNA and one-base mismatched miRNA. Conclusions The novel nanomaterial MoS2-based fluorescence sensor has the advantages of fast,high sensitivity and specificity,and it is potential for the detection of breast cancer-related miRNA.