吸烟与种植体周围炎龈沟液中IL-17A表达的相关性研究

Correlation between smoking and IL-17A expression in peri-implant sulcus fluid of patients with peri-implantitis

目的:研究吸烟对种植体周围龈沟液(PICF)中IL-17A表达的影响。方法:将种植体周围炎患者分为吸烟组和非吸烟组,并将种植体对侧健康天然牙设为对照组。检测牙周临床指标改良牙龈指数(mGI)、改良龈沟出血指数(mSBI)、探诊深度(PD),并根据X线片测定牙槽骨吸收程度(aBL);采集龈沟液,运用ELISA试验检测IL-17A的浓度。结果:吸烟组IL-17A浓度高于其对照组(P<0.05);吸烟对照组IL-17A浓度高于非吸烟对照组(P<0.05);吸烟组IL-17A浓度高于非吸烟组(P<0.05)。吸烟组和非吸烟组IL-17A浓度与PICF体积量有正相关性,吸烟组IL-17A浓度与PD、aBL成正相关,与mGI和mSBI成负相关。结论:IL-17A可作为种植体周围炎的标志性炎症因子之一,吸烟可导致IL-17A表达的升高,推测吸烟是引起种植体周围炎的危险因素之一。

AIM： To explore the effects of smoking on IL-17A expression in peri-implant sulcus fluid （PISF） of patients with peri-implantitis. METHODS： The patients were divided into smoking group（ n = 13） and non-smoking group（n = 13）. Implant site group of the subjects was included into implant group and the contralateral natural teeth group was included into control group. Modified gingival index （ mGI） , modified sulcus bleeding index （mSBI） , probing depth（PD） ,alveolar bone loss（aBL）were detected, and the gingival sulcus fluid was collected. The concentration of IL-17A was measured by ELISA. RESULTS： In smoking group, the IL-17A concentration in implant group was higher than that in the control group （P 〈0. 05）. Both implant sites and contralateral natural teeth had sig-nificantly higher expression of IL-17A in smoking group than that in nonsmoking group （P 〈0. 0 5 ） . In both smoking and nonsmoking groups, IL-17A concentration was positively correlated with PISF volume. Furthermore, IL-17A con-centration in smoking group was positively correlated with AL and PD, and negatively correlated with mGI and mSBI. CONCLUSION： Smoking can lead to increased expression of IL-17A, IL-17A can be used as one of inflammatory markers in peri-implantitis. It is speculated that smoking is one of the risk factors of peri-implantitis.