植物乳杆菌SCP53生物膜的形成条件

Study on biofilm formation conditions of Lactobacillus plantarum SCP53

研究了不同状态下植物乳杆菌SPC53生物膜形成能力的情况。通过改变其生长的环境条件与营养状况,采用结晶紫染色法和扫描电镜(scanning electron microscope,SEM)观察,定量及定性分析其生物膜的形成。结果表明:接种量5×10~7CFU/mL,培养时间36 h,培养温度42℃,pH值5.8是植物乳杆菌SCP53生物膜形成的最适培养条件,在MRS肉汤中依次添加200 mg/mL NaCl、75 mg/mL葡萄糖、7.5 mg/mL果胶、75 mg/mL全脂乳粉,可以促进植物乳杆菌生物膜的形成,形成后的生物膜A_(490)值为4.492 3。对照组A_(490)值为0.136 5,扫描电镜结果显示生物膜与浮游菌体有明显差异。

The biofilm formation ability of Lactobacillus plantarum SCP53 under different status was studied here- in. The biofilm formed by Lactobacillus plantarum under different environmental conditions and nutrition had been quantitatively and qualitatively evaluated through determination of crystal violet stain in OD490 and scanning electron microscope（SEM）. The results showed that the optimum culture condition for biofihn formation of Lactobacillus plan- tarum SCP53 was incubation at 42℃ for 36 h with inoculums size of 5 × 107 CFU/mL and pH value of 5.8. Addition of NaC1 （200 mg/mL）, glucose （75 mg/mL）, pectin （7.5 mg/mL）, milk powder （75 mg/mL） in MRS could pro- mote biofilm formation of Lactobacillus plantarum. Biofilms A490 values of formed biofilm was 4. 492 3, while this value in control group was 0. 136 5. Results from scanning electron microscopy （SEM） showed that there was obvious difference between the biological membrane and planktonic bacteria.