利用产量位点标记分析中国水稻骨干恢复系与南亚及东南亚恢复系的遗传多样性

Genetic Diversity Analysis of Core Rice Restorer Lines of China and South Asia and Southeat Asia Based on Yield Loci Related Markers

利用47个根据已报道的QTL位点或者已被精细定位或克隆的与水稻产量性状基因紧密连锁的产量位点标记,对来自中国、印度和越南等国的58份水稻恢复系进行遗传多样性分析。结果表明:(1)在中国恢复系中,47个产量位点标记中有36个具有多态性,共检测到90个等位基因,每个标记检测到等位基因2~4个,平均为2.500个;有效等位基因共62.905个,平均每个标记1.747个;36个有效标记的Shannon信息指数平均值为0.632,变幅为0.271~1.266。(2)在来自国外的材料中,47个产量位点标记均具有多态性,共检测到131个等位基因,每个标记检测到的等位基因数为2~6个,平均2.787个;有效等位基因数共82.686个,平均1.759个;47个标记的Shannon信息指数平均值为0.649,变幅为0.109~1.110。(3)聚类分析显示,在遗传相似系数为0.73水平上,参试资源聚为三大类群,中国资源多聚在第Ⅰ类群下的第1、2、3亚群,越南资源多聚在第Ⅰ-4亚群,孟加拉资源多聚在第Ⅲ-3亚群。由此表明,中国资源遗传基础较为狭窄,而其他国家的恢复系具有较远的亲缘关系。

The objective of this study is to analyze the genetic diversity of 58 varieties of rice restorer lines based on yield loci related markers. 47 yield loci related markers were used to scan 58 varieties from China and south and southeast Asia. The result showed：（ 1 ）In restorer lines from China,36 yield loci related markers showed polymorphism, and 90 alleles were detected with an average of 2. 500 alleles per marker （62. 905 effective alleles with an average of 1. 747 alleles per marker） , and the average of Shannon information index was 0.632, ranged from 0.271 - 1. 266. （2） In restorer lines from another countries, all of the 47 yield loci related markers showed polymor- phism,and totally 131 alleles were detected with an average of 2.787 alleles per marker（82. 686 effective alleles with an average of 1. 759 alleles per marker） , and the average of Shannon information index was 0. 649, ranged from 0. 109-1.110. （3）Cluster analysis showed that the 58 varieties could be clustered into three categories on the level of 0.73 genetic similarity coefficient. The varieties from China were mainly clustered in 1,2,3 subsets of group I .Vietnamese varieties were mainly clustered in 4 subsets of group I , Bangladesh varieties were mainly clustered in 4 subsets of group Ⅲ. All of above results showed a narrow genetic base in the tested Chinese varieties and Chinese varieties had a distant genetic relationship to other countries varieties.