摘要
利用SRAP分子标记对从各主要产地收集到的90份薏苡种质进行遗传多样性分析,其中68份收集于福建省,6份来自中国台湾,16份来自浙江、辽宁、山东、河南、云南、江苏、湖南、广东、上海等省(市)。结果表明,从88对SRAP引物组合中筛选出26对引物进行SRAP扩增,共扩增出185条带,其中具有多态性的有157,占总数的84.86%,表明90份薏苡种质表现出丰富的遗传多样性。基于SRAP标记利用系统聚类法将90份薏苡种质资源分为4大类,与形态性状分类结果有一定的相似性;利用16对SRAP引物构建了73份薏苡种质资源的DNA指纹图谱,为薏苡遗传研究、品种选育与资源保护提供了依据。
Genetic diversity of 90 Coix lacryma-jobi L. germplasm resources collected from the main places of production were analyzed by Sequence-related amplified polymorphism (SRAP)marker, including 68 collected from Fujian, 6 from Taiwan, and another 16 from Zhejiang, Liaoning, Shandong, Henan, Yunnan, Jiangsu, Hunan, Guang- dong, Shanghai etc. It showed that 26 pairs of primers were selected from 88 pairs of SRAP primers. 26 pairs of SRAP primers produced 185 amplified fragments, of which 157 were polymorphic, accounting for 84.67% of the to- tal. It showed that there was distinct genetic diversity among 90 Coix lacryma-jobi L. germplasm resources. 90 Coix lacryma-jobi L. germplasm resources were classified into four categories basing on the UPGMA cluster analysis, which was similar to the result by morphological markers. A DNA fingerprint with 73 Coix lacryma-jobi L. was con- structed by 16 pairs of SRAP primers which was significant for genetic research, variety breeding and resource pro- tection of Coix lacryma-jobi.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2017年第3期413-420,共8页
Journal of Plant Genetic Resources
基金
青年骨干教师研修项目(201507870009)
福建省科技厅重点项目(2011N0004)
福建省教育厅项目(JA10111)
福建省自然科学基金指导性科技计划项目(2012D087)
关键词
薏苡
种质资源
SRAP标记
DNA指纹图谱
Coix lacryma-jobi L.
germplasm resources
SRAP marker
DNA fingerprint