斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F_1的GISH分析

GISH Analysis of Intergeneric Complex between Erianthus arundinaceus and Saccharum spontaneum(GXAS07-6-1) and Its F_1 Hybrids from Crosses with Sugarcane(Saccharum spp.)

斑茅割手密复合体(GXAS07-6-1)是广西蔗茅属斑茅和广西甘蔗属割手密的属间杂种,聚集了双亲的优点。本研究利用基于Alu-like的PCR鉴定方法对GXAS07-6-1及甘蔗与GXAS07-6-1的3份F_1材料进行真实性鉴定,基于基因组原位杂交技术(GISH)对父本GXAS07-6-1及其3份F_1染色体组成及核型进行分析。研究结果表明:3份F_1材料为GXAS07-6-1的真杂种;父本GXAS07-6-1的染色体众数为62条,其中30条来自蔗茅属斑茅,32条来自甘蔗属割手密,核型分类属于1B,其染色体按"n+n"方式传递;GXASF_108-2-17、GXASF_108-2-22、GXASF_108-2-32的染色体数目为78~80条,其中69~71条来自甘蔗属,9~11条来自蔗茅属斑茅,3份F_1的核型分类分别属于2B、1B、1B,染色体传递方式均为"n+n"。父本GXAS07-6-1及3份F_1材料中均未发现有染色体的交换或易位现象。甘蔗与斑割复合体杂交,蔗茅属斑茅染色体在亲子间传递过程存在丢失现象。

GXAS07-6-1, an intergeneric hybrid of Erianthus arundinaceus （ Retz. ） Jeswiet and Saccharum spon- taneum L. has the advantages of its parents. In this study, the turth and fales of three F1 hybrids progenies GXAS08- 2-17, GXAS08-2-22 and GXAS08-2-32 were identified by the PCR method of Alu-like. The chromosomes composi-tion and karyotype of the GXAS07-6-1 and the F1 generation were analyzed using the genomic in situ hybridization （GISH） technique. The results showed that the three F1 materials were true progenies. There were 62 chromosomes in GXAS07-6-1 ,of which 30 were from E. arundinaceus （Retz.）Jeswiet and 32 were from S. spontaneum L.. and the somatic chromosome karyotypic was 1 B, following n ＋ n transmission. Furthermore, the chromosomes numbers of GXASF108-2-17, GXASFI08-2-22 and GXASF108-2-32 ranged from 78 to 80,of which 69 -71 were from Saccha- rum spp. and 9 to 11 were from E. arundinaceus （ Retz. ） Jeswiet . The somatic chromosome karyotypes of three Fl progeny were 2B, 1B, 1B, respectively, and all of the three progenies following n ＋ n transmission. Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and its F1 hybrids as well Chromosome missing also was confirmed from E. arundinaceus （Retz.）Jeswiet in the F1 progeny.